Overview
- External ontology
- TOGO:M2927 — KM2 cell-free medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2927 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009462["km2_cell_free_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009462 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Calf_brains["Calf brains"]
CultureMech_009462 --> ing1_Calf_brains
ing2_Beef_heart["Beef heart"]
CultureMech_009462 --> ing2_Beef_heart
ing3_Proteose_peptone["Proteose peptone"]
CultureMech_009462 --> ing3_Proteose_peptone
ing4_Dextrose["Dextrose"]
CultureMech_009462 --> ing4_Dextrose
CHEBI_17634["CHEBI:17634"]
ing4_Dextrose -.-> CHEBI_17634
ing5_Sodium_chloride["Sodium chloride"]
CultureMech_009462 --> ing5_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing5_Sodium_chloride -.-> CHEBI_26710
ing6_Disodium_phosphate["Disodium phosphate"]
CultureMech_009462 --> ing6_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing6_Disodium_phosphate -.-> CHEBI_34683
ing7_PPLO__Difco_["PPLO (Difco)"]
CultureMech_009462 --> ing7_PPLO__Difco_
ing8_Pig_serum__Invitrogen_["Pig serum (Invitrogen)"]
CultureMech_009462 --> ing8_Pig_serum__Invitrogen_
ing9_Horse_serum__Invitrogen_["Horse serum (Invitrogen)"]
CultureMech_009462 --> ing9_Horse_serum__Invitrogen_
ing10_100_mg_ml_azlocillin["100 mg/ml azlocillin"]
CultureMech_009462 --> ing10_100_mg_ml_azlocillin
ing11_100_mg_ml_flucloxacillin["100 mg/ml flucloxacillin"]
CultureMech_009462 --> ing11_100_mg_ml_flucloxacillin
ing12_1_0_M_NaOH["1.0 M NaOH"]
CultureMech_009462 --> ing12_1_0_M_NaOH
ing13_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009462 --> ing13_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_7H2O -.-> CHEBI_31795
ing14_NaCl["NaCl"]
CultureMech_009462 --> ing14_NaCl
ing14_NaCl -.-> CHEBI_26710
ing15_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009462 --> ing15_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing15_CaCl2_2H2O -.-> CHEBI_86158
ing16_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_009462 --> ing16_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing16_MgCl2_6H2O -.-> CHEBI_86345
ing17_KCl["KCl"]
CultureMech_009462 --> ing17_KCl
CHEBI_32588["CHEBI:32588"]
ing17_KCl -.-> CHEBI_32588
ing18_KH2PO4["KH2PO4"]
CultureMech_009462 --> ing18_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing18_KH2PO4 -.-> CHEBI_63036
ing19_Na2HPO4_12H2O["Na2HPO4·12H2O"]
CultureMech_009462 --> ing19_Na2HPO4_12H2O
ing19_Na2HPO4_12H2O -.-> CHEBI_34683
ing20_0_1_M_NaOH["0.1 M NaOH"]
CultureMech_009462 --> ing20_0_1_M_NaOH
ing21_Phenol_red["Phenol red"]
CultureMech_009462 --> ing21_Phenol_red
CHEBI_31991["CHEBI:31991"]
ing21_Phenol_red -.-> CHEBI_31991
ing22_azlocillin["azlocillin"]
CultureMech_009462 --> ing22_azlocillin
ing23_flucloxacillin["flucloxacillin"]
CultureMech_009462 --> ing23_flucloxacillin
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
369.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 365.0, 1.0, 1.0, 1.0, 1.0] |
| 2 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 3 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 4 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 5 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 6 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 7 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 8 | PPLO (Difco) | 1.6 G_PER_L | Properties: Complex component, Undefined component | |
| 9 | Pig serum (Invitrogen) | 50 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 10 | Horse serum (Invitrogen) | 50 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 11 | 100 mg/ml azlocillin | 25 G_PER_L | ||
| 12 | 100 mg/ml flucloxacillin | 25 G_PER_L | ||
| 13 | 1.0 M NaOH | variable VARIABLE | Properties: Defined component, Inorganic compound, Solution | |
| 14 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
NaCl
Cl.Na 58.443 g/mol
|
160 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.7 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
KCl
Cl.K 74.551 g/mol
|
4 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Na2HPO4·12H2O
HO4P.2Na 141.958 g/mol
|
3 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 21 | 0.1 M NaOH | 10 G_PER_L | Properties: Defined component, Inorganic compound, Solution | |
| 22 |
Phenol red
C19H14O5S 354.383 g/mol
|
0.6 PERCENT_W_V |
CHEBI:31991
phenol red
|
Role: pH indicator; Properties: Organic compound, Defined component, Simple component |
| 23 | azlocillin | 100 G_PER_L | Role: Antimicrobial agent; Properties: Defined component, Simple component | |
| 24 | flucloxacillin | 100 G_PER_L | Role: Antimicrobial agent; Properties: Defined component, Simple component |
Solutions
Fresh baker's yeast extract solution
Dose: 18 G_PER_L
Properties: Undefined component
Sterile solution A
Dose: 12.5 G_PER_L
Sterile solution B
Dose: 12.5 G_PER_L
Phenol red solution
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- km2_cell_free_medium (CultureMech:009462)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.409448Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2927 |
| 2026-01-28T19:48:06.700980Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 1.0 M NaOH |
| 2026-03-09T08:03:32.269635+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:08:37.355561Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:06.273741Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009462 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.249212+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "KM2 cell-free medium" to "km2_cell_free_medium" for consistent matching |
| 2026-05-16T09:03:38.505601+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:10.423110+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:50.095644+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:00.374389+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:39.634226+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 71ca657885f11099b7945709d60542f33e365eac1cddc9108d730ad8de5de71a |