Overview
- External ontology
- TOGO:M294 — RC+ Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M294 Original source: JCM - JCM_M299 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=299
Composition
flowchart LR
CultureMech_009476["rc_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009476 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_009476 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009476 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_009476 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_009476 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_009476 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_009476 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009476 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_Na2S_9H2O["Na2S・9H2O"]
CultureMech_009476 --> ing8_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_9H2O -.-> CHEBI_76209
ing9_KCl["KCl"]
CultureMech_009476 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10_Sodium_acetate["Sodium acetate"]
CultureMech_009476 --> ing10_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing10_Sodium_acetate -.-> CHEBI_32954
ing11_MES["MES"]
CultureMech_009476 --> ing11_MES
CHEBI_39010["CHEBI:39010"]
ing11_MES -.-> CHEBI_39010
ing12_L__Cystine["L--Cystine"]
CultureMech_009476 --> ing12_L__Cystine
ing13_Maltose_monohydrate["Maltose monohydrate"]
CultureMech_009476 --> ing13_Maltose_monohydrate
ing14_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009476 --> ing14_Yeast_extract__BD_Difco_
ing15_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_009476 --> ing15_Trypticase_peptone__BD_BBL_
ing16_H2SO4["H2SO4"]
CultureMech_009476 --> ing16_H2SO4
CHEBI_26836["CHEBI:26836"]
ing16_H2SO4 -.-> CHEBI_26836
ing17_N2["N2"]
CultureMech_009476 --> ing17_N2
CHEBI_17997["CHEBI:17997"]
ing17_N2 -.-> CHEBI_17997
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.83 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 12 | MES | 2.13 G_PER_L |
CHEBI:39010
MES
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 13 | L--Cystine | 12 G_PER_L | Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component | |
| 14 | Maltose monohydrate | 7.2 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component | |
| 15 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 16 | Trypticase peptone (BD-BBL) | 5 G_PER_L | Properties: Undefined component | |
| 17 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 18 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M294_RC_Medium (CultureMech:009476)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.782864Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M294 |
| 2026-01-28T19:48:22.290584Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: N2 |
| 2026-03-13T06:20:06.325473Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009476 |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.301752+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RC+ Medium" to "rc_medium" for consistent matching |
| 2026-05-16T09:03:16.271914+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:39.540923+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.011869+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:14.123780+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c80634e2c9c8663479cfa0774f1b7221128a865bf0e02d66e64257e0c610f912 |