Overview
- External ontology
- TOGO:M2952 — nutrient-rich A medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2952
Composition
flowchart LR
CultureMech_009479["nutrient_rich_a_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009479 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4___7H2O["MgSO4 · 7H2O"]
CultureMech_009479 --> ing1_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4___7H2O -.-> CHEBI_31795
ing2_yeast_extract["yeast extract"]
CultureMech_009479 --> ing2_yeast_extract
ing3_KH2PO4["KH2PO4"]
CultureMech_009479 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_K2HPO4["K2HPO4"]
CultureMech_009479 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_FeSO4___7H2O["FeSO4 · 7H2O"]
CultureMech_009479 --> ing5_FeSO4___7H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4___7H2O -.-> CHEBI_75836
ing6_biotin["biotin"]
CultureMech_009479 --> ing6_biotin
CHEBI_15956["CHEBI:15956"]
ing6_biotin -.-> CHEBI_15956
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_009479 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_MnSO4___H2O["MnSO4 · H2O"]
CultureMech_009479 --> ing8_MnSO4___H2O
CHEBI_86364["CHEBI:86364"]
ing8_MnSO4___H2O -.-> CHEBI_86364
ing9_thiamine["thiamine"]
CultureMech_009479 --> ing9_thiamine
CHEBI_18385["CHEBI:18385"]
ing9_thiamine -.-> CHEBI_18385
ing10_chloramphenicol__if_needed_["chloramphenicol (if needed)"]
CultureMech_009479 --> ing10_chloramphenicol__if_needed_
ing11_kanamycin__if_needed_["kanamycin (if needed)"]
CultureMech_009479 --> ing11_kanamycin__if_needed_
ing12_glucose["glucose"]
CultureMech_009479 --> ing12_glucose
CHEBI_17234["CHEBI:17234"]
ing12_glucose -.-> CHEBI_17234
ing13_gentamicin__if_needed_["gentamicin (if needed)"]
CultureMech_009479 --> ing13_gentamicin__if_needed_
ing14_casamino_acid["casamino acid"]
CultureMech_009479 --> ing14_casamino_acid
ing15__NH2_2CO["(NH2)2CO"]
CultureMech_009479 --> ing15__NH2_2CO
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
MgSO4 · 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
FeSO4 · 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
6 MG_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
biotin
C10H16N2O3S 244.316 g/mol
|
0.2 MG_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
7 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
MnSO4 · H2O
H2O.Mn.O4S 169.016 g/mol
|
4.2 MG_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
thiamine
C12H17N4OS 265.362 g/mol
|
0.2 MG_PER_L |
CHEBI:18385
thiamine(1+)
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 11 | chloramphenicol (if needed) | 5 G_PER_L | Role: Antimicrobial agent; Properties: Organic compound, Defined component, Simple component | |
| 12 | kanamycin (if needed) | 50 G_PER_L | Role: Antimicrobial agent; Properties: Organic compound, Defined component, Simple component | |
| 13 |
glucose
C6H12O6 180.156 g/mol
|
4 PERCENT_W_V |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 14 | gentamicin (if needed) | 50 G_PER_L | Role: Antimicrobial agent; Properties: Organic compound, Defined component, Simple component | |
| 15 | casamino acid | 7 G_PER_L | Properties: Undefined component | |
| 16 | (NH2)2CO | 2 G_PER_L | Properties: Defined component, Simple component, Organic compound |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- nutrient_rich_a_medium (CultureMech:009479)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.421541Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2952 |
| 2026-01-28T19:48:28.270375Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:06.342101Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009479 |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.323570+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "nutrient-rich A medium" to "nutrient_rich_a_medium" for consistent matching |
| 2026-06-05T07:41:40.238445+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.449434+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:58:28.519751+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:09.702884+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:51.803904+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 39ac0dee327608da256cfafc76e2b50fce0c5dc5fca4bbd2a4fcec0303cc5f9a |