Overview
- External ontology
- TOGO:M2970 — brain heart infusion (BHI) medium (supplemented with antibiotics)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2970 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009493["brain_heart_infusion_bhi_medium_supplemented_with_antibiotics"]
ing0_kanamycin["kanamycin"]
CultureMech_009493 --> ing0_kanamycin
ing1_ampicillin["ampicillin"]
CultureMech_009493 --> ing1_ampicillin
CHEBI_28971["CHEBI:28971"]
ing1_ampicillin -.-> CHEBI_28971
ing2_erythromycin["erythromycin"]
CultureMech_009493 --> ing2_erythromycin
CHEBI_48923["CHEBI:48923"]
ing2_erythromycin -.-> CHEBI_48923
ing3_chloramphenicol["chloramphenicol"]
CultureMech_009493 --> ing3_chloramphenicol
ing4_Calf_brains["Calf brains"]
CultureMech_009493 --> ing4_Calf_brains
ing5_Beef_heart["Beef heart"]
CultureMech_009493 --> ing5_Beef_heart
ing6_Proteose_peptone["Proteose peptone"]
CultureMech_009493 --> ing6_Proteose_peptone
ing7_Dextrose["Dextrose"]
CultureMech_009493 --> ing7_Dextrose
CHEBI_17634["CHEBI:17634"]
ing7_Dextrose -.-> CHEBI_17634
ing8_Sodium_chloride["Sodium chloride"]
CultureMech_009493 --> ing8_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing8_Sodium_chloride -.-> CHEBI_26710
ing9_Disodium_phosphate["Disodium phosphate"]
CultureMech_009493 --> ing9_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing9_Disodium_phosphate -.-> CHEBI_34683
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | kanamycin | 50 G_PER_L | Role: Antimicrobial agent; Properties: Defined component, Organic compound, Solution | |
| 2 |
ampicillin
C16H19N3O4S 349.412 g/mol
|
50 G_PER_L |
CHEBI:28971
ampicillin
|
Role: Antimicrobial agent; Properties: Organic compound, Solution, Defined component |
| 3 | erythromycin | 30 G_PER_L |
CHEBI:48923
erythromycin
|
Role: Antimicrobial agent; Properties: Solution, Defined component, Complex component |
| 4 | chloramphenicol | 50 G_PER_L | Role: Antimicrobial agent | |
| 5 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 6 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 7 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 8 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 9 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 10 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- brain_heart_infusion_bhi_medium_supplemented_with_antibiotics (CultureMech:009493)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.432653Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2970 |
| 2026-01-28T19:48:11.053342Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:06.404954Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009493 |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.404436+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "brain heart infusion (BHI) medium (supplemented with antibiotics)" to "brain_heart_infusion_bhi_medium_supplemented_with_antibiotics" for consistent matching |
| 2026-06-05T07:40:51.477282+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:25.879175+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 435adfdeb90615c08d4d777b2ebd020a7b8933ac06da0981da3160e71d2ce9ae |