Overview
- External ontology
- TOGO:M2976 — brain-heart infusion broth (supplemented with hemin and β-NAD)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2976
Composition
flowchart LR
CultureMech_009499["brain_heart_infusion_broth_supplemented_with_hemin_and_nad"]
ing0_hemin["hemin"]
CultureMech_009499 --> ing0_hemin
CHEBI_50385["CHEBI:50385"]
ing0_hemin -.-> CHEBI_50385
ing1___NAD["β-NAD"]
CultureMech_009499 --> ing1___NAD
ing2_Difco_brain_heart_infusion_broth__BD_Biosciences_["Difco brain-heart infusion broth (BD Biosciences)"]
CultureMech_009499 --> ing2_Difco_brain_heart_infusion_broth__BD_Biosciences_
ing3_CO2["CO2"]
CultureMech_009499 --> ing3_CO2
CHEBI_16526["CHEBI:16526"]
ing3_CO2 -.-> CHEBI_16526
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
hemin
C34H32ClFeN4O4 651.952 g/mol
|
10 G_PER_L |
CHEBI:50385
hemin
|
Role: Growth factor; Properties: Defined component, Organic compound, Simple component |
| 2 | β-NAD | 10 G_PER_L | Role: Growth factor; Properties: Defined component, Simple component, Organic compound | |
| 3 | Difco brain-heart infusion broth (BD Biosciences) | 1 G_PER_L | Properties: Undefined component, Complex component | |
| 4 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- brain_heart_infusion_broth_supplemented_with_hemin_and_nad (CultureMech:009499)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.436928Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2976 |
| 2026-01-28T19:48:08.648981Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2 |
| 2026-03-13T06:20:06.419741Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009499 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.420107+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "brain-heart infusion broth (supplemented with hemin and β-NAD)" to "brain_heart_infusion_broth_supplemented_with_hemin_and_nad" for consistent matching |
| 2026-05-16T09:03:26.185052+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:51.507602+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:25.921506+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7f68ce53dd354b912d97a37359e1eb3a3675c36743eff3e38f95561679ed75d8 |