Overview
- External ontology
- TOGO:M2978 — MOPS (morpholinethanesulfonic acid) minimal medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2978
Composition
flowchart LR
CultureMech_009501["mops_morpholinethanesulfonic_acid_minimal_medium"]
ing0_NaCl["NaCl"]
CultureMech_009501 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_NH4Cl["NH4Cl"]
CultureMech_009501 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_K2HPO4["K2HPO4"]
CultureMech_009501 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_MOPS["MOPS"]
CultureMech_009501 --> ing3_MOPS
CHEBI_39074["CHEBI:39074"]
ing3_MOPS -.-> CHEBI_39074
ing4_MgCl2["MgCl2"]
CultureMech_009501 --> ing4_MgCl2
CHEBI_6636["CHEBI:6636"]
ing4_MgCl2 -.-> CHEBI_6636
ing5_K2SO4["K2SO4"]
CultureMech_009501 --> ing5_K2SO4
CHEBI_32036["CHEBI:32036"]
ing5_K2SO4 -.-> CHEBI_32036
ing6_FeSO4["FeSO4"]
CultureMech_009501 --> ing6_FeSO4
CHEBI_75832["CHEBI:75832"]
ing6_FeSO4 -.-> CHEBI_75832
ing7_Tricine["Tricine"]
CultureMech_009501 --> ing7_Tricine
CHEBI_46760["CHEBI:46760"]
ing7_Tricine -.-> CHEBI_46760
ing8_CaCl2["CaCl2"]
CultureMech_009501 --> ing8_CaCl2
CHEBI_3312["CHEBI:3312"]
ing8_CaCl2 -.-> CHEBI_3312
ing9_Micronutrients["Micronutrients"]
CultureMech_009501 --> ing9_Micronutrients
ing10_H3BO3["H3BO3"]
CultureMech_009501 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_CuSO4["CuSO4"]
CultureMech_009501 --> ing11_CuSO4
CHEBI_23414["CHEBI:23414"]
ing11_CuSO4 -.-> CHEBI_23414
ing12_CoCl2["CoCl2"]
CultureMech_009501 --> ing12_CoCl2
CHEBI_35696["CHEBI:35696"]
ing12_CoCl2 -.-> CHEBI_35696
ing13_MnCl2["MnCl2"]
CultureMech_009501 --> ing13_MnCl2
CHEBI_63041["CHEBI:63041"]
ing13_MnCl2 -.-> CHEBI_63041
ing14_ZnSO4["ZnSO4"]
CultureMech_009501 --> ing14_ZnSO4
CHEBI_35176["CHEBI:35176"]
ing14_ZnSO4 -.-> CHEBI_35176
ing15__NH4_6_MO7_24["(NH4)6(MO7)24"]
CultureMech_009501 --> ing15__NH4_6_MO7_24
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
50 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
9.52 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.32 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 4 | MOPS | 40 MILLIMOLAR |
CHEBI:39074
MOPS
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 5 |
MgCl2
2Cl.Mg 95.211 g/mol
|
0.523 MILLIMOLAR |
CHEBI:6636
magnesium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
K2SO4
2K.O4S 174.259 g/mol
|
0.276 MILLIMOLAR |
CHEBI:32036
potassium sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 |
FeSO4
Fe.O4S 151.908 g/mol
|
0.01 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 8 | Tricine | 4 MILLIMOLAR |
CHEBI:46760
tricine
|
Properties: Buffer |
| 9 |
CaCl2
Ca.2Cl 110.984 g/mol
|
variable VARIABLE |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 10 | Micronutrients | variable VARIABLE | ||
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
variable VARIABLE |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 12 |
CuSO4
Cu.O4S 159.609 g/mol
|
variable VARIABLE |
CHEBI:23414
copper(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 13 |
CoCl2
2Cl.Co 129.839 g/mol
|
variable VARIABLE |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 14 |
MnCl2
Cl2Mn 125.844 g/mol
|
variable VARIABLE |
CHEBI:63041
manganese(II) chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 15 |
ZnSO4
O4S.Zn 161.453 g/mol
|
variable VARIABLE |
CHEBI:35176
zinc sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 16 | (NH4)6(MO7)24 | variable VARIABLE | Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mops_morpholinethanesulfonic_acid_minimal_medium (CultureMech:009501)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.438327Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2978 |
| 2026-01-28T19:48:28.559367Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CaCl2; Added default concentration for ingredient: Micronutrients; Added default concentration for ingredient: H3BO3; Added default concentration for ingredient: CuSO4; Added default concentration for ingredient: CoCl2; Added default concentration for ingredient: MnCl2; Added default concentration for ingredient: ZnSO4; Added default concentration for ingredient: (NH4)6(MO7)24 |
| 2026-02-06T05:10:37.943745Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:20:06.428883Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009501 |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.429119+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MOPS (morpholinethanesulfonic acid) minimal medium" to "mops_morpholinethanesulfonic_acid_minimal_medium" for consistent matching |
| 2026-05-16T09:04:26.625130+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:38.268518+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.114381+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:50.224148+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c6bfccee65f5847645bcb0890a35d02e95c709c641e97030fdacdbebfb455f45 |