Overview
- External ontology
- TOGO:M298 — PE Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M298 Original source: JCM - JCM_M303 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=303
Composition
flowchart LR
CultureMech_009509["pe_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009509 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_009509 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_Sodium_acetate["Sodium acetate"]
CultureMech_009509 --> ing2_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing2_Sodium_acetate -.-> CHEBI_32954
ing3_Sodium_succinate["Sodium succinate"]
CultureMech_009509 --> ing3_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing3_Sodium_succinate -.-> CHEBI_63675
ing4_Sodium_glutamate["Sodium glutamate"]
CultureMech_009509 --> ing4_Sodium_glutamate
CHEBI_64220["CHEBI:64220"]
ing4_Sodium_glutamate -.-> CHEBI_64220
ing5_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009509 --> ing5_Yeast_extract__BD_Difco_
ing6_Na2S2O3["Na2S2O3"]
CultureMech_009509 --> ing6_Na2S2O3
CHEBI_32150["CHEBI:32150"]
ing6_Na2S2O3 -.-> CHEBI_32150
ing7_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_009509 --> ing7_Casamino_acids__BD_Difco_
ing8_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009509 --> ing8_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_7H2O -.-> CHEBI_31795
ing9_NaCl["NaCl"]
CultureMech_009509 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
ing10_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009509 --> ing10_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_2H2O -.-> CHEBI_86158
ing11_FeSO4_H2O["FeSO4・H2O"]
CultureMech_009509 --> ing11_FeSO4_H2O
CHEBI_75834["CHEBI:75834"]
ing11_FeSO4_H2O -.-> CHEBI_75834
ing12_EDTA_3Na["EDTA・3Na"]
CultureMech_009509 --> ing12_EDTA_3Na
CHEBI_63125["CHEBI:63125"]
ing12_EDTA_3Na -.-> CHEBI_63125
ing13_KH2PO4["KH2PO4"]
CultureMech_009509 --> ing13_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing13_KH2PO4 -.-> CHEBI_63036
ing14_K2HPO4["K2HPO4"]
CultureMech_009509 --> ing14_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing14_K2HPO4 -.-> CHEBI_131527
ing15_Biotin["Biotin"]
CultureMech_009509 --> ing15_Biotin
CHEBI_15956["CHEBI:15956"]
ing15_Biotin -.-> CHEBI_15956
ing16_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
CultureMech_009509 --> ing16_p__Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing16_p__Aminobenzoic_acid -.-> CHEBI_30753
ing17_Thiamine_HCl["Thiamine・HCl"]
CultureMech_009509 --> ing17_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing17_Thiamine_HCl -.-> CHEBI_49105
ing18_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_009509 --> ing18_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing18_Calcium_pantothenate -.-> CHEBI_31345
ing19_Pyridoxine_HCl["Pyridoxine・HCl"]
CultureMech_009509 --> ing19_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing19_Pyridoxine_HCl -.-> CHEBI_30961
ing20_Folic_acid["Folic acid"]
CultureMech_009509 --> ing20_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing20_Folic_acid -.-> CHEBI_27470
ing21_Vitamin_B12["Vitamin B12"]
CultureMech_009509 --> ing21_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing21_Vitamin_B12 -.-> CHEBI_176843
ing22_Nicotinic_acid["Nicotinic acid"]
CultureMech_009509 --> ing22_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing22_Nicotinic_acid -.-> CHEBI_15940
ing23_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_009509 --> ing23_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_2H2O -.-> CHEBI_75213
ing24_H3BO3["H3BO3"]
CultureMech_009509 --> ing24_H3BO3
CHEBI_33118["CHEBI:33118"]
ing24_H3BO3 -.-> CHEBI_33118
ing25_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_009509 --> ing25_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing25_ZnSO4_7H2O -.-> CHEBI_32312
ing26_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_009509 --> ing26_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing26_CuSO4_5H2O -.-> CHEBI_31440
ing27_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_009509 --> ing27_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing27_MnSO4_xH2O -.-> CHEBI_86360
ing28_Co_NO3_2_6H2O["Co(NO3)2・6H2O"]
CultureMech_009509 --> ing28_Co_NO3_2_6H2O
CHEBI_86214["CHEBI:86214"]
ing28_Co_NO3_2_6H2O -.-> CHEBI_86214
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
603.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 1.0, 1.0, 1.0, 100.0, 500.0] |
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.5 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 4 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
0.5 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 5 |
Sodium glutamate
C5H8NO4.Na 169.112 g/mol
|
0.5 G_PER_L |
CHEBI:64220
monosodium glutamate
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 6 | Yeast extract (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 7 |
Na2S2O3
5H2O.2Na.O3S2 248.186 g/mol
|
0.5 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Simple component, Inorganic compound |
| 8 | Casamino acids (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 9 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
24.65 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
23.4 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
2.94 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
FeSO4・H2O
Fe.H2O.O4S 169.923 g/mol
|
1.11 G_PER_L |
CHEBI:75834
iron(2+) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
EDTA・3Na
C10H13N2O8.3Na 358.19 g/mol
|
224.72 G_PER_L |
CHEBI:63125
EDTA trisodium salt
|
Properties: Simple component, Organic compound, Defined component [Merged 3 duplicates: 4.12, 200.0, 20.6] |
| 14 |
KH2PO4
H2O4P.K 136.084 g/mol
|
75 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
K2HPO4
HO4P.2K 174.174 g/mol
|
78 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
Biotin
C10H16N2O3S 244.316 g/mol
|
5 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 |
p--Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
50 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
100 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
50 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
|
50 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
Folic acid
C19H19N7O6 441.404 g/mol
|
50 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 | Vitamin B12 | 1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
100 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
1.21 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
H3BO3
H3BO3 61.833 g/mol
|
1.55 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.44 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
1.26 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
5.58 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
Co(NO3)2・6H2O
Co.6H2O.2NO3 291.031 g/mol
|
1.46 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Basal salts solution (see below)
Dose: 5 G_PER_L
Phosphate solution (see below)
Dose: 5 G_PER_L
Vitamin solution (see below)
Dose: 1 G_PER_L
Trace elements solution (see below)
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M298_PE_Medium (CultureMech:009509)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.791665Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M298 |
| 2026-01-28T19:48:27.682231Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:45.802682+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:16.305027Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 6 duplicate ingredient(s) |
| 2026-03-13T06:20:06.463111Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009509 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.466977+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PE Medium" to "pe_medium" for consistent matching |
| 2026-05-16T09:03:16.329697+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:39.590772+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:33.812729+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 1 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:28.996695+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.028106+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:14.193214+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ede756c730c87a87407dd881fea685642c1f3b55692d4bcbfe0e1a5426b27105 |