Overview
- External ontology
- TOGO:M299 — PYGV Marine Medium (A)
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M299 Original source: JCM - JCM_M304 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=304
Composition
flowchart LR
CultureMech_009516["pygv_marine_medium_a"]
ing0_Agar["Agar"]
CultureMech_009516 --> ing0_Agar
CHEBI_2509["CHEBI:2509"]
ing0_Agar -.-> CHEBI_2509
ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009516 --> ing1_Yeast_extract__BD_Difco_
ing2_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
CultureMech_009516 --> ing2_Bacto_peptone__BD_Difco_
ing3_Artificial_seawater__see_below_["Artificial seawater (see below)"]
CultureMech_009516 --> ing3_Artificial_seawater__see_below_
ing4_KOH["KOH"]
CultureMech_009516 --> ing4_KOH
CHEBI_32035["CHEBI:32035"]
ing4_KOH -.-> CHEBI_32035
ing5_Distilled_water["Distilled water"]
CultureMech_009516 --> ing5_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing5_Distilled_water -.-> CHEBI_15377
ing6_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009516 --> ing6_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_7H2O -.-> CHEBI_31795
ing7_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009516 --> ing7_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_2H2O -.-> CHEBI_86158
ing8_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_009516 --> ing8_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing8_Na2MoO4_2H2O -.-> CHEBI_75213
ing9_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_009516 --> ing9_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_7H2O -.-> CHEBI_75836
ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009516 --> ing10_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
ing11_Biotin["Biotin"]
CultureMech_009516 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
CultureMech_009516 --> ing12_p__Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing12_p__Aminobenzoic_acid -.-> CHEBI_30753
ing13_Thiamine_HCl["Thiamine・HCl"]
CultureMech_009516 --> ing13_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing13_Thiamine_HCl -.-> CHEBI_49105
ing14_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_009516 --> ing14_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing14_Calcium_pantothenate -.-> CHEBI_31345
ing15_Pyridoxine_HCl["Pyridoxine・HCl"]
CultureMech_009516 --> ing15_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing15_Pyridoxine_HCl -.-> CHEBI_30961
ing16_Folic_acid["Folic acid"]
CultureMech_009516 --> ing16_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing16_Folic_acid -.-> CHEBI_27470
ing17_Vitamin_B12["Vitamin B12"]
CultureMech_009516 --> ing17_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing17_Vitamin_B12 -.-> CHEBI_176843
ing18_Riboflavin["Riboflavin"]
CultureMech_009516 --> ing18_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing18_Riboflavin -.-> CHEBI_17015
ing19_Nicotinamide["Nicotinamide"]
CultureMech_009516 --> ing19_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing19_Nicotinamide -.-> CHEBI_17154
ing20_NaCl["NaCl"]
CultureMech_009516 --> ing20_NaCl
CHEBI_26710["CHEBI:26710"]
ing20_NaCl -.-> CHEBI_26710
ing21_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009516 --> ing21_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing21_MgCl2_6H2O -.-> CHEBI_86345
ing22_H3BO3["H3BO3"]
CultureMech_009516 --> ing22_H3BO3
CHEBI_33118["CHEBI:33118"]
ing22_H3BO3 -.-> CHEBI_33118
ing23_KCl["KCl"]
CultureMech_009516 --> ing23_KCl
CHEBI_32588["CHEBI:32588"]
ing23_KCl -.-> CHEBI_32588
ing24_NaHCO3["NaHCO3"]
CultureMech_009516 --> ing24_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing24_NaHCO3 -.-> CHEBI_32139
ing25_Na2SO4["Na2SO4"]
CultureMech_009516 --> ing25_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing25_Na2SO4 -.-> CHEBI_32149
ing26_SrCl2_6H2O["SrCl2・6H2O"]
CultureMech_009516 --> ing26_SrCl2_6H2O
CHEBI_36385["CHEBI:36385"]
ing26_SrCl2_6H2O -.-> CHEBI_36385
ing27_NaF["NaF"]
CultureMech_009516 --> ing27_NaF
CHEBI_28741["CHEBI:28741"]
ing27_NaF -.-> CHEBI_28741
ing28_KBr["KBr"]
CultureMech_009516 --> ing28_KBr
CHEBI_32030["CHEBI:32030"]
ing28_KBr -.-> CHEBI_32030
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 2 | Yeast extract (BD-Difco) | 0.25 G_PER_L | Properties: Undefined component | |
| 3 | Bacto peptone (BD-Difco) | 0.25 G_PER_L | Properties: Undefined component | |
| 4 | Artificial seawater (see below) | 960 G_PER_L | ||
| 5 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 6 |
Distilled water
H2O 18.015 g/mol
|
952.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 950.0, 1.0, 1.0] |
| 7 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
29.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
4.442 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 3.34, 1.102] |
| 9 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
13 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
99 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 13 |
p--Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
5 G_PER_L |
CHEBI:17154
nicotinamide
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 21 |
NaCl
Cl.Na 58.443 g/mol
|
23.477 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.981 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
H3BO3
H3BO3 61.833 g/mol
|
26 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
KCl
Cl.K 74.551 g/mol
|
664 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
NaHCO3
CHO3.Na 84.006 g/mol
|
192 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.917 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
SrCl2・6H2O
Cl2Sr.6H2O 266.616 g/mol
|
24 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
NaF
F.Na 41.988 g/mol
|
3 G_PER_L |
CHEBI:28741
sodium fluoride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
KBr
Br.K 119.002 g/mol
|
6 G_PER_L |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
2.5% Glucose solution
Dose: 10 G_PER_L
Role: Carbon source; Properties: Defined component, Organic compound, Solution
Mineral salt solution (see below)
Dose: 20 G_PER_L
Vitamin solution (see below)
Dose: 10 G_PER_L
Metals ''44'' (see Medium [M140])
Dose: 50 G_PER_L
Cross-reference to Medium M140
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pygv_marine_medium_a (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.795844Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M299 |
| 2026-01-28T19:48:23.203411Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH; Added default concentration for ingredient: KOH |
| 2026-03-09T08:04:38.577774+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:08.370631Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:06.514215Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009516 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.521826+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYGV Marine Medium (A)" to "pygv_marine_medium_a" for consistent matching |
| 2026-05-16T09:04:29.881016+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:43.160454+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.943597+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 1 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:58:30.379363+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:10.615543+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:54.000845+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d895831928a23ee49e934017dc59ade52cf4498e749de79fd0564567c8787905 |