modified_bn_medium_for_m08dhb (CultureMech:009526)

Overview

External ontology

TOGO:M3009 — Modified BN Medium For M08dhb

Applications

Microbial cultivation

Synonyms

{'name': 'methanomethylovorans_uponensis_medium', 'source': 'TOGO', 'source_id': 'TOGO:M1038', 'original_category': 'archaea'}
{'name': 'geobacter_daltonii_medium', 'source': 'TOGO', 'source_id': 'TOGO:M781', 'original_category': 'bacterial'}

Notes

Source: https://togomedium.org/medium/M3009 Original source: JCM - JCM_M1337 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1337

Composition

flowchart LR
    CultureMech_009526["modified_bn_medium_for_m08dhb"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009526 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_009526 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_009526 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_009526 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_009526 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Resazurin["Resazurin"]
    CultureMech_009526 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_009526 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_KCl["KCl"]
    CultureMech_009526 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009526 --> ing8_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing8_Carbon_dioxide_gas -.-> CHEBI_16526
    ing9_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009526 --> ing9_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing9_Nitrogen_gas -.-> CHEBI_17997

Ingredients (10)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	960 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2	NaCl Cl.Na 58.443 g/mol	1 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3	CaCl2·2H2O Ca.2Cl.2H2O 147.014 g/mol	0.1 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
4	KH2PO4 H2O4P.K 136.084 g/mol	0.2 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
5	NH4Cl Cl.H4N 53.492 g/mol	0.5 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
6	Resazurin C12H7NO4 229.191 g/mol	1 G_PER_L	CHEBI:8806 Resazurin	Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
7	MgCl2·6H2O 2Cl.6H2O.Mg 203.301 g/mol	0.4 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
8	KCl Cl.K 74.551 g/mol	0.5 G_PER_L	CHEBI:32588 potassium chloride	Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
9	Carbon dioxide gas CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Properties: ガス
10	Nitrogen gas N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: ガス

Solutions

Trace elements solution (see Medium [M1094])

Dose: 1 G_PER_L

Cross-reference to Medium M1094

8% NaHCO3 solution*

Dose: 25 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

1.0% yeast extract solution

Dose: 5 G_PER_L

Role: Nutrient source; Properties: Undefined component

5% Na2S·9H2O solution

Dose: 10 G_PER_L

Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution

1.0 M Sodium thiosulfate solution

Dose: 10 G_PER_L

Properties: Defined component, Inorganic compound, Solution

1.0 M Sodium formate solution

Dose: 10 G_PER_L

Properties: Defined component, Solution, Organic compound

Trace vitamins (see Medium [M190])

Dose: 1 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

Merged from 3 source records.

TOGO_M1038_Methanomethylovorans_Uponensis_Medium (CultureMech:007553)
TOGO_M781_Geobacter_Daltonii_Medium (CultureMech:010190)
modified_bn_medium_for_m08dhb (CultureMech:009526)

Timestamp	Curator	Action	Notes
2026-01-27T06:35:53.678547Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M3009
2026-01-28T19:48:30.658470Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:51.019059+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 7 solution(s) from ingredients
2026-03-13T06:20:06.600215Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009526
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:30.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:25.614377+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Modified BN Medium For M08dhb" to "modified_bn_medium_for_m08dhb" for consistent matching
2026-05-16T09:04:25.194566+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:41:36.363375+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.573905+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:24.434319+00:00	recipe-merger	Merged 3 duplicate recipes into canonical record	Sources: TOGO_M1038_Methanomethylovorans_Uponensis_Medium, TOGO_M781_Geobacter_Daltonii_Medium, modified_bn_medium_for_m08dhb
2026-08-06T03:04:24.434377+00:00	merge_recipes.py	MERGED_RECIPES	merged 3 source recipe(s) on fingerprint 330432bf555bfb3d42ef54b34eda236a3b0c2cc369fe73f9ebf19049d63b210b