methanococcoides_medium_2

CultureMech:009543 archaea COMPLEX LIQUID
Original name: Methanococcoides Medium 2

Overview

External ontology
TOGO:M3031 — Methanococcoides Medium 2
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M3031 Original source: JCM - JCM_M1396 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1396

Composition

flowchart LR
    CultureMech_009543["methanococcoides_medium_2"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009543 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_009543 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_009543 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_009543 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_009543 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Resazurin["Resazurin"]
    CultureMech_009543 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_009543 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_KCl["KCl"]
    CultureMech_009543 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_NaHCO3["NaHCO3"]
    CultureMech_009543 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_Fe_NH4_2_SO4_2_6H2O["Fe(NH4)2(SO4)2·6H2O"]
    CultureMech_009543 --> ing9_Fe_NH4_2_SO4_2_6H2O
    CHEBI_76181["CHEBI:76181"]
    ing9_Fe_NH4_2_SO4_2_6H2O -.-> CHEBI_76181
    ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009543 --> ing10_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing10_Carbon_dioxide_gas -.-> CHEBI_16526
    ing11_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009543 --> ing11_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing11_Nitrogen_gas -.-> CHEBI_17997
    ing12_N2["N2"]
    CultureMech_009543 --> ing12_N2
    ing12_N2 -.-> CHEBI_17997

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
9 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.14 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.14 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Defined component, Inorganic compound
5 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
6 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component
7 MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
4 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
8 KCl
Cl.K 74.551 g/mol
0.34 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
9 NaHCO3
CHO3.Na 84.006 g/mol
5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
10 Fe(NH4)2(SO4)2·6H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
2 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
11 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
12 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
13 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス

Solutions

Wolfe's mineral solution (see Medium [M257])

Dose: 10 G_PER_L

Cross-reference to Medium M257

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

50% (v/v) Methanol solution*

Dose: 10 G_PER_L

Role: Solvating media; Properties: Defined component, Organic compound, Solution

3% Trimethylamine solution*

Dose: 10 G_PER_L

Role: Nutrient source

5% Na2S·9H2O solution

Dose: 10 G_PER_L

Role: Reducing agent; Properties: Inorganic compound, Solution, Defined component

5% L-Cysteine·HCl·H2O solution

Dose: 10 G_PER_L

Properties: Defined component, Organic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:53.703603Z togo-import Imported from TOGO Medium Source: JCM, ID: M3031
2026-01-28T19:48:37.456532Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2
2026-03-09T08:05:02.468992+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 6 solution(s) from ingredients
2026-03-13T06:20:06.731988Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009543
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:25.756366+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Methanococcoides Medium 2" to "methanococcoides_medium_2" for consistent matching
2026-05-16T09:04:21.674206+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:41:31.177413+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.843032+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:30.057533+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint c1b7cb06b52e227a10412b0abf4977404f1be03636e44f2f90ee09bb9c11a1e9