Overview
- External ontology
- TOGO:M3033 — Modified YCFA Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3033 Original source: JCM - JCM_M1407 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1407
Composition
flowchart LR
CultureMech_009545["modified_ycfa_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009545 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Resazurin["Resazurin"]
CultureMech_009545 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_NaHCO3["NaHCO3"]
CultureMech_009545 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_Cellobiose["Cellobiose"]
CultureMech_009545 --> ing3_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing3_Cellobiose -.-> CHEBI_17057
ing4_Maltose["Maltose"]
CultureMech_009545 --> ing4_Maltose
CHEBI_18167["CHEBI:18167"]
ing4_Maltose -.-> CHEBI_18167
ing5_Glucose["Glucose"]
CultureMech_009545 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Yeast_extract__BD__Difco_["Yeast extract (BD--Difco)"]
CultureMech_009545 --> ing6_Yeast_extract__BD__Difco_
ing7_Tryptone__BD__Difco_["Tryptone (BD--Difco)"]
CultureMech_009545 --> ing7_Tryptone__BD__Difco_
ing8_L_Cysteine_HCl_H2O["L-Cysteine·HCl·H2O"]
CultureMech_009545 --> ing8_L_Cysteine_HCl_H2O
ing9_K2HPO4["K2HPO4"]
CultureMech_009545 --> ing9_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing9_K2HPO4 -.-> CHEBI_131527
ing10_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009545 --> ing10_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing10_MgSO4_7H2O -.-> CHEBI_31795
ing11_NaCl["NaCl"]
CultureMech_009545 --> ing11_NaCl
CHEBI_26710["CHEBI:26710"]
ing11_NaCl -.-> CHEBI_26710
ing12_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009545 --> ing12_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_2H2O -.-> CHEBI_86158
ing13_KH2PO4["KH2PO4"]
CultureMech_009545 --> ing13_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing13_KH2PO4 -.-> CHEBI_63036
ing14__NH4_2SO4["(NH4)2SO4"]
CultureMech_009545 --> ing14__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing14__NH4_2SO4 -.-> CHEBI_62946
ing15_10_N_NaOH["10 N NaOH"]
CultureMech_009545 --> ing15_10_N_NaOH
ing16_Acetic_acid["Acetic acid"]
CultureMech_009545 --> ing16_Acetic_acid
CHEBI_15366["CHEBI:15366"]
ing16_Acetic_acid -.-> CHEBI_15366
ing17_n__Valeric_acid["n--Valeric acid"]
CultureMech_009545 --> ing17_n__Valeric_acid
ing18_iso__Valeric_acid["iso--Valeric acid"]
CultureMech_009545 --> ing18_iso__Valeric_acid
ing19_iso__Butyric_acid["iso--Butyric acid"]
CultureMech_009545 --> ing19_iso__Butyric_acid
ing20_Propionic_acid["Propionic acid"]
CultureMech_009545 --> ing20_Propionic_acid
CHEBI_30768["CHEBI:30768"]
ing20_Propionic_acid -.-> CHEBI_30768
ing21_Ethanol["Ethanol"]
CultureMech_009545 --> ing21_Ethanol
CHEBI_16236["CHEBI:16236"]
ing21_Ethanol -.-> CHEBI_16236
ing22_Hemin["Hemin"]
CultureMech_009545 --> ing22_Hemin
CHEBI_50385["CHEBI:50385"]
ing22_Hemin -.-> CHEBI_50385
ing23_KOH["KOH"]
CultureMech_009545 --> ing23_KOH
CHEBI_32035["CHEBI:32035"]
ing23_KOH -.-> CHEBI_32035
ing24_Biotin["Biotin"]
CultureMech_009545 --> ing24_Biotin
CHEBI_15956["CHEBI:15956"]
ing24_Biotin -.-> CHEBI_15956
ing25_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
CultureMech_009545 --> ing25_p__Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing25_p__Aminobenzoic_acid -.-> CHEBI_30753
ing26_Pyridoxine_HCl["Pyridoxine・HCl"]
CultureMech_009545 --> ing26_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing26_Pyridoxine_HCl -.-> CHEBI_30961
ing27_Folic_acid["Folic acid"]
CultureMech_009545 --> ing27_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing27_Folic_acid -.-> CHEBI_27470
ing28_Vitamin_B12["Vitamin B12"]
CultureMech_009545 --> ing28_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing28_Vitamin_B12 -.-> CHEBI_176843
ing29_Thiamine_HCl["Thiamine・HCl"]
CultureMech_009545 --> ing29_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing29_Thiamine_HCl -.-> CHEBI_49105
CultureMech_009545 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1762.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 6 duplicates: 660.0, 1.0, 1.0, 100.0, 500.0, 500.0] |
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Cellobiose
C12H22O11 342.297 g/mol
|
2 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 5 |
Maltose
C12H22O11 342.297 g/mol
|
2 G_PER_L |
CHEBI:18167
alpha-maltose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
2 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 7 | Yeast extract (BD--Difco) | 2.5 G_PER_L | Properties: Undefined component | |
| 8 | Tryptone (BD--Difco) | 10 G_PER_L | Properties: Undefined component | |
| 9 | L-Cysteine·HCl·H2O | 1 G_PER_L | Properties: Defined component, Simple component, Organic compound | |
| 10 |
K2HPO4
HO4P.2K 174.174 g/mol
|
3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.6 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
NaCl
Cl.Na 58.443 g/mol
|
6 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
6 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 16 | 10 N NaOH | 26 G_PER_L | Properties: Defined component, Inorganic compound, Solution | |
| 17 |
Acetic acid
C2H4O2 60.052 g/mol
|
17 G_PER_L |
CHEBI:15366
acetic acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 18 | n--Valeric acid | 1 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component | |
| 19 | iso--Valeric acid | 1 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component | |
| 20 | iso--Butyric acid | 1 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component | |
| 21 |
Propionic acid
C3H6O2 74.079 g/mol
|
6 G_PER_L |
CHEBI:30768
propionic acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 22 |
Ethanol
C2H6O 46.069 g/mol
|
25 G_PER_L |
CHEBI:16236
ethanol
|
Role: Solvating media; Properties: Organic compound, Defined component, Simple component |
| 23 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
0.1 G_PER_L |
CHEBI:50385
hemin
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 24 |
KOH
HO.K 56.105 g/mol
|
0.28 G_PER_L |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Biotin
C10H16N2O3S 244.316 g/mol
|
5 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 |
p--Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
15 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 |
Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
|
75 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Folic acid
C19H19N7O6 441.404 g/mol
|
25 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 | Vitamin B12 | 5 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
25 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
25 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
Mineral solution I (see Medium [M1210])
Dose: 150 G_PER_L
Cross-reference to Medium M1210
Mineral solution II (see Medium [M1210])
Dose: 150 G_PER_L
Cross-reference to Medium M1210
VFA mix (see Medium [M1210])
Dose: 6.2 G_PER_L
Cross-reference to Medium M1210
Hemin solution (see Medium [M1210])
Dose: 10 G_PER_L
Cross-reference to Medium M1210
Vitamin solution I (see Medium [M1210])
Dose: 1 G_PER_L
Cross-reference to Medium M1210
Vitamin solution II (see Medium [M1210])
Dose: 1 G_PER_L
Cross-reference to Medium M1210
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_ycfa_medium (CultureMech:009545)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.708971Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M3033 |
| 2026-01-28T19:48:38.703016Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:04.806026+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:09:36.769559Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:20:06.762618Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009545 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.786922+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified YCFA Medium" to "modified_ycfa_medium" for consistent matching |
| 2026-05-16T09:04:26.495414+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:38.079184+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.189953+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 1 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:58:27.016767+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:09.054549+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:50.094998+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1199fe822b9d4644e0d0304393b7fcebcaa2651cf7cbe3fd3af47e100ceb4a79 |