r2a_fumarate_anaerobic

CultureMech:009555 bacterial COMPLEX LIQUID
Original name: R2A + fumarate (anaerobic)

Overview

External ontology
TOGO:M3042 — R2A + fumarate (anaerobic)
Applications
  • Microbial cultivation
pH range
{'min': 6.0, 'max': 7.0}
Notes
Source: https://togomedium.org/medium/M3042 Original source: NBRC - NBRC_M1520-2 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1520

Composition

flowchart LR
    CultureMech_009555["r2a_fumarate_anaerobic"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009555 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_009555 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_K2HPO4["K2HPO4"]
    CultureMech_009555 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_009555 --> ing3_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing3_Sodium_pyruvate -.-> CHEBI_50144
    ing4_Glucose["Glucose"]
    CultureMech_009555 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Soluble_starch["Soluble starch"]
    CultureMech_009555 --> ing5_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing5_Soluble_starch -.-> CHEBI_28017
    ing6_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
    CultureMech_009555 --> ing6_Bacto_Yeast_Extract__Difco_
    ing7_Bacto_Proteose_Peptone_No__3__Difco_["Bacto Proteose Peptone No. 3 (Difco)"]
    CultureMech_009555 --> ing7_Bacto_Proteose_Peptone_No__3__Difco_
    ing8_Sodium_fumarate["Sodium fumarate"]
    CultureMech_009555 --> ing8_Sodium_fumarate
    CHEBI_115156["CHEBI:115156"]
    ing8_Sodium_fumarate -.-> CHEBI_115156
    ing9_Bacto_Casamino_Acids__Difco_["Bacto Casamino Acids (Difco)"]
    CultureMech_009555 --> ing9_Bacto_Casamino_Acids__Difco_
    ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009555 --> ing10_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing10_Carbon_dioxide_gas -.-> CHEBI_16526
    ing11_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009555 --> ing11_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing11_Nitrogen_gas -.-> CHEBI_17997

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
4 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.3 G_PER_L CHEBI:50144
sodium pyruvate
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
5 Glucose
C6H12O6 180.156 g/mol
0.5 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
6 Soluble starch 0.5 G_PER_L CHEBI:28017
starch
 Role: Carbon source; Properties: Undefined component
7 Bacto Yeast Extract (Difco) 0.5 G_PER_L Properties: Undefined component
8 Bacto Proteose Peptone No. 3 (Difco) 0.5 G_PER_L Properties: Undefined component
9 Sodium fumarate
C4H2O4.2Na 160.036 g/mol
1.4 G_PER_L CHEBI:115156
disodium fumarate
 Properties: Organic compound, Simple component, Defined component
10 Bacto Casamino Acids (Difco) 0.5 G_PER_L Properties: Undefined component
11 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 NBRC Medium 1520 uses an N2/CO2 atmosphere for anaerobic liquid dispensing; retained as a variable gas-phase component.
12 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 NBRC Medium 1520 uses an N2/CO2 atmosphere for anaerobic liquid dispensing; retained as a variable gas-phase component.

Preparation

  1. Dispense the liquid medium under an N2/CO2 atmosphere.
  2. Seal the dispensed medium with butyl rubber stoppers.
  3. Autoclave at 121 C for 20 min.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:59.226011Z togo-import Imported from TOGO Medium Source: NBRC, ID: M3042
2026-01-28T19:48:38.521788Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-13T06:20:06.870859Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009555
2026-03-17T06:30:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:25.894951+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "R2A + fumarate (anaerobic)" to "r2a_fumarate_anaerobic" for consistent matching
2026-05-16T09:03:16.551661+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:39.819325+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:29.217298+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:50.105945+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-30T00:00:00-07:00 repair_togo_nbrc_water_ph_review_batch.py RESOLVED_TOGO_M3042_NBRC1520_REVIEW Corrected TOGO/NBRC Distilled water from the imported 1 g/L to the source 1 L final-volume row, restored the NBRC 1520 pH 6-7 range, preserved the N2/CO2 butyl-stopper autoclave instruction, and linked TOGO M3041 as the solid physical-state variant.
2026-09-30T20:37:21.910707+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 4622984f1f66edbabae7a0659e3d96c062d9e03e2da9c87ce050cfb74b93d768