Overview
- External ontology
- TOGO:M3043 — Medium for Methylotrophs
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3043 Original source: NBRC - NBRC_M1563 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1563
Composition
flowchart LR
CultureMech_009556["medium_for_methylotrophs"]
ing0_Distilled_water["Distilled water"]
CultureMech_009556 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009556 --> ing1_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_2H2O -.-> CHEBI_86158
ing2_KNO3["KNO3"]
CultureMech_009556 --> ing2_KNO3
CHEBI_63043["CHEBI:63043"]
ing2_KNO3 -.-> CHEBI_63043
ing3_Methanol["Methanol"]
CultureMech_009556 --> ing3_Methanol
CHEBI_17790["CHEBI:17790"]
ing3_Methanol -.-> CHEBI_17790
ing4_MgSO4_6H2O["MgSO4·6H2O"]
CultureMech_009556 --> ing4_MgSO4_6H2O
CHEBI_32599["CHEBI:32599"]
ing4_MgSO4_6H2O -.-> CHEBI_32599
ing5_NaCl["NaCl"]
CultureMech_009556 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_009556 --> ing6_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing6_Na2MoO4_2H2O -.-> CHEBI_75213
ing7_H3BO3["H3BO3"]
CultureMech_009556 --> ing7_H3BO3
CHEBI_33118["CHEBI:33118"]
ing7_H3BO3 -.-> CHEBI_33118
ing8_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_009556 --> ing8_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_4H2O -.-> CHEBI_86368
ing9_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_009556 --> ing9_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing9_CoCl2_6H2O -.-> CHEBI_35696
ing10_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_009556 --> ing10_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing10_NiCl2_6H2O -.-> CHEBI_34887
ing11_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_009556 --> ing11_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing11_CuCl2_2H2O -.-> CHEBI_86318
ing12_ZnCl2["ZnCl2"]
CultureMech_009556 --> ing12_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing12_ZnCl2 -.-> CHEBI_49976
ing13_FeCl3_6H2O["FeCl3·6H2O"]
CultureMech_009556 --> ing13_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing13_FeCl3_6H2O -.-> CHEBI_86254
ing14_Nitrilotriacetic_acid__NTA_["Nitrilotriacetic acid (NTA)"]
CultureMech_009556 --> ing14_Nitrilotriacetic_acid__NTA_
CHEBI_44557["CHEBI:44557"]
ing14_Nitrilotriacetic_acid__NTA_ -.-> CHEBI_44557
ing15_NaOH["NaOH"]
CultureMech_009556 --> ing15_NaOH
CHEBI_32145["CHEBI:32145"]
ing15_NaOH -.-> CHEBI_32145
ing16_KH2PO4["KH2PO4"]
CultureMech_009556 --> ing16_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing16_KH2PO4 -.-> CHEBI_63036
ing17_Na2HPO4_12H2O["Na2HPO4·12H2O"]
CultureMech_009556 --> ing17_Na2HPO4_12H2O
CHEBI_34683["CHEBI:34683"]
ing17_Na2HPO4_12H2O -.-> CHEBI_34683
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
3.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 1.0] |
| 2 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.30000000000000004 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.2, 0.1] |
| 3 |
KNO3
K.NO3 101.102 g/mol
|
1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Methanol
CH4O 32.042 g/mol
|
10 G_PER_L |
CHEBI:17790
methanol
|
Role: Solvating media; Properties: Organic compound, Defined component, Simple component |
| 5 |
MgSO4·6H2O
Mg.O4S 120.368 g/mol
|
1 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.25 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.024 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
0.12 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.025 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.35 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Nitrilotriacetic acid (NTA)
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 16 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 17 |
KH2PO4
H2O4P.K 136.084 g/mol
|
26 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Na2HPO4·12H2O
HO4P.2Na 141.958 g/mol
|
71.6 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace element solution*
Dose: 1 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Phosphate buffer**
Dose: 10 G_PER_L
See mediadive_5249_Phosphate_buffer.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- medium_for_methylotrophs (CultureMech:009556)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.227568Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M3043 |
| 2026-01-28T19:48:27.612941Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:45.698073+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:16.182082Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:06.881957Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009556 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.905210+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium for Methylotrophs" to "medium_for_methylotrophs" for consistent matching |
| 2026-05-16T09:04:20.099591+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:29.200290+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.094338+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:43.193037+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d8e83871bb1b3239052bb2b7ffd2b61d48c4c3664b0a7376465f999f33fe636a |