Overview
- External ontology
- TOGO:M3045 — Brain Heart Infusion (BHI) with Sodium Acetate
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3045 Original source: NBRC - NBRC_M1574-2 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1574 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009558["brain_heart_infusion_bhi_with_sodium_acetate"]
ing0_Distilled_water["Distilled water"]
CultureMech_009558 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Sodium_acetate["Sodium acetate"]
CultureMech_009558 --> ing1_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing1_Sodium_acetate -.-> CHEBI_32954
ing2_Calf_brains["Calf brains"]
CultureMech_009558 --> ing2_Calf_brains
ing3_Beef_heart["Beef heart"]
CultureMech_009558 --> ing3_Beef_heart
ing4_Proteose_peptone["Proteose peptone"]
CultureMech_009558 --> ing4_Proteose_peptone
ing5_Dextrose["Dextrose"]
CultureMech_009558 --> ing5_Dextrose
CHEBI_17634["CHEBI:17634"]
ing5_Dextrose -.-> CHEBI_17634
ing6_Sodium_chloride["Sodium chloride"]
CultureMech_009558 --> ing6_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing6_Sodium_chloride -.-> CHEBI_26710
ing7_Disodium_phosphate["Disodium phosphate"]
CultureMech_009558 --> ing7_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing7_Disodium_phosphate -.-> CHEBI_34683
ing8_N2["N2"]
CultureMech_009558 --> ing8_N2
CHEBI_17997["CHEBI:17997"]
ing8_N2 -.-> CHEBI_17997
ing9_Antifoam_SI__Wako_["Antifoam SI (Wako)"]
CultureMech_009558 --> ing9_Antifoam_SI__Wako_
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
10.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 1.0, 9.0] |
| 2 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
2.7 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 3 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 4 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 5 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 6 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 7 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 8 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 9 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 10 | Antifoam SI (Wako) | 1 G_PER_L | Role: Defoaming agent; Properties: Defined component |
Solutions
Antifoam solution (only for liquid medium)*
Dose: 2.5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M3045_Brain_Heart_Infusion_BHI_with_Sodium_Acetate (CultureMech:009558)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.231416Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M3045 |
| 2026-01-28T19:48:11.581167Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:18.992108+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:46.768595Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:06.895064Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009558 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.918852+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Brain Heart Infusion (BHI) with Sodium Acetate" to "brain_heart_infusion_bhi_with_sodium_acetate" for consistent matching |
| 2026-05-16T09:03:16.561496+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:39.828965+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:14.447648+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 01d8d23a32cc5f85ea251ed17cadbe923d78da4a1861ce9b3a3877dafdc9a514 |