Overview
- External ontology
- TOGO:M3049 — MRS + 20 % sucrose
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3049 Original source: NBRC - NBRC_M1599 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1599
Composition
flowchart LR
CultureMech_009562["mrs_20_sucrose"]
ing0_Distilled_water["Distilled water"]
CultureMech_009562 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009562 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009562 --> ing2_Yeast_extract
ing3_K2HPO4["K2HPO4"]
CultureMech_009562 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Sodium_acetate["Sodium acetate"]
CultureMech_009562 --> ing4_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing4_Sodium_acetate -.-> CHEBI_32954
ing5_Tween_80["Tween 80"]
CultureMech_009562 --> ing5_Tween_80
CHEBI_53426["CHEBI:53426"]
ing5_Tween_80 -.-> CHEBI_53426
ing6_MnSO4_nH2O["MnSO4·nH2O"]
CultureMech_009562 --> ing6_MnSO4_nH2O
CHEBI_86360["CHEBI:86360"]
ing6_MnSO4_nH2O -.-> CHEBI_86360
ing7_Diammonium_hydrogen_citrate["Diammonium hydrogen citrate"]
CultureMech_009562 --> ing7_Diammonium_hydrogen_citrate
CHEBI_63076["CHEBI:63076"]
ing7_Diammonium_hydrogen_citrate -.-> CHEBI_63076
ing8_Gellan_gum__if_needed_["Gellan gum (if needed)"]
CultureMech_009562 --> ing8_Gellan_gum__if_needed_
ing9_Sucrose["Sucrose"]
CultureMech_009562 --> ing9_Sucrose
CHEBI_17992["CHEBI:17992"]
ing9_Sucrose -.-> CHEBI_17992
ing10_Meat_extract["Meat extract"]
CultureMech_009562 --> ing10_Meat_extract
ing11_Peptone["Peptone"]
CultureMech_009562 --> ing11_Peptone
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 3 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
5 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 6 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Defined component, Simple component, Organic compound |
| 7 |
MnSO4·nH2O
Mn.O4S 151.001 g/mol
|
0.05 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
Diammonium hydrogen citrate
C6H6O7.2H4N 226.185 g/mol
|
2 G_PER_L |
CHEBI:63076
diammonium citrate
|
Role: Nutrient source; Properties: Organic compound, Defined component, Simple component |
| 9 | Gellan gum (if needed) | 8 G_PER_L | Role: Solidifying component; Properties: Organic compound, Undefined component | |
| 10 |
Sucrose
C12H22O11 342.297 g/mol
|
200 G_PER_L |
CHEBI:17992
sucrose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 11 | Meat extract | 10 G_PER_L | Properties: Undefined component | |
| 12 | Peptone | 10 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mrs_20_sucrose (CultureMech:009562)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.236512Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M3049 |
| 2026-01-28T19:48:36.236339Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:06.919117Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009562 |
| 2026-03-17T06:30:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.942994+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MRS + 20 % sucrose" to "mrs_20_sucrose" for consistent matching |
| 2026-06-05T07:41:38.391233+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:27.227074+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:09.161671+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:50.385607+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 674aac9286d3a1f525b60a31ddf654be85504d340fff4668c978bb2d5d903c5a |