Overview
- External ontology
- TOGO:M3053 — PY4S medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3053 Original source: NBRC - NBRC_M1606 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1606
Composition
flowchart LR
CultureMech_009567["py4s_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009567 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009567 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_009567 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009567 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009567 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_009567 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_009567 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_009567 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_Na2CO3["Na2CO3"]
CultureMech_009567 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_Cellobiose["Cellobiose"]
CultureMech_009567 --> ing9_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing9_Cellobiose -.-> CHEBI_17057
ing10_Maltose["Maltose"]
CultureMech_009567 --> ing10_Maltose
CHEBI_18167["CHEBI:18167"]
ing10_Maltose -.-> CHEBI_18167
ing11_Glucose["Glucose"]
CultureMech_009567 --> ing11_Glucose
CHEBI_17234["CHEBI:17234"]
ing11_Glucose -.-> CHEBI_17234
ing12_Soluble_starch["Soluble starch"]
CultureMech_009567 --> ing12_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing12_Soluble_starch -.-> CHEBI_28017
ing13_Agar__if_needed_["Agar (if needed)"]
CultureMech_009567 --> ing13_Agar__if_needed_
ing14_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_009567 --> ing14_Bacto_Yeast_Extract__Difco_
ing15_Cysteine_HCl["Cysteine-HCl"]
CultureMech_009567 --> ing15_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing15_Cysteine_HCl -.-> CHEBI_91247
ing16_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
CultureMech_009567 --> ing16_Bacto_Tryptone__Difco_
ing17_N2["N2"]
CultureMech_009567 --> ing17_N2
CHEBI_17997["CHEBI:17997"]
ing17_N2 -.-> CHEBI_17997
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.09 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.9 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.09 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.45 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.45 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound |
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.9 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.2 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.25 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 11 |
Maltose
C12H22O11 342.297 g/mol
|
0.25 G_PER_L |
CHEBI:18167
alpha-maltose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 12 |
Glucose
C6H12O6 180.156 g/mol
|
0.25 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Defined component, Organic compound |
| 13 | Soluble starch | 0.25 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 14 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 15 | Bacto Yeast Extract (Difco) | 5 G_PER_L | Properties: Undefined component | |
| 16 |
Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
|
0.3 G_PER_L |
CHEBI:91247
L-cysteine hydrochloride
|
Properties: Organic compound, Simple component, Defined component |
| 17 | Bacto Tryptone (Difco) | 10 G_PER_L | Properties: Undefined component | |
| 18 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- py4s_medium (CultureMech:009567)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.243877Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M3053 |
| 2026-01-28T19:48:24.832384Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-13T06:20:06.960839Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009567 |
| 2026-03-17T06:30:17.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.984194+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PY4S medium" to "py4s_medium" for consistent matching |
| 2026-05-16T09:04:29.696215+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:42.894032+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:30.205853+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:10.535344+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:53.819399+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 522ccb70f60ff86e264f818a423b796a3efc54f5ba264dc4abaea9af556bd01b |