Overview
- External ontology
- TOGO:M3115 — Nitrogen-limited mineral salts medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3115
Composition
flowchart LR
CultureMech_009587["nitrogen_limited_mineral_salts_medium"]
ing0_MgSO4_7H2O["MgSO4.7H2O"]
CultureMech_009587 --> ing0_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_7H2O -.-> CHEBI_31795
ing1_KH2PO4["KH2PO4"]
CultureMech_009587 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_009587 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Na2HPO4_12H2O["Na2HPO4.12H2O"]
CultureMech_009587 --> ing3_Na2HPO4_12H2O
CHEBI_34683["CHEBI:34683"]
ing3_Na2HPO4_12H2O -.-> CHEBI_34683
ing4_Deionized_water["Deionized water"]
CultureMech_009587 --> ing4_Deionized_water
ing5_0_1_M_HCl["0.1 M HCl"]
CultureMech_009587 --> ing5_0_1_M_HCl
ing6_CoCl2_6H2O["CoCl2.6H2O"]
CultureMech_009587 --> ing6_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing6_CoCl2_6H2O -.-> CHEBI_35696
ing7_NiCl3_6H2O["NiCl3.6H2O"]
CultureMech_009587 --> ing7_NiCl3_6H2O
ing8_CuSO4_5H2O["CuSO4.5H2O"]
CultureMech_009587 --> ing8_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing8_CuSO4_5H2O -.-> CHEBI_31440
ing9_CaCl2["CaCl2"]
CultureMech_009587 --> ing9_CaCl2
CHEBI_3312["CHEBI:3312"]
ing9_CaCl2 -.-> CHEBI_3312
ing10_FeCl3["FeCl3"]
CultureMech_009587 --> ing10_FeCl3
CHEBI_30808["CHEBI:30808"]
ing10_FeCl3 -.-> CHEBI_30808
ing11_CrCl3_6H2O["CrCl3.6H2O"]
CultureMech_009587 --> ing11_CrCl3_6H2O
ing12_Fructose["Fructose"]
CultureMech_009587 --> ing12_Fructose
CHEBI_28645["CHEBI:28645"]
ing12_Fructose -.-> CHEBI_28645
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4.7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
2 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
Na2HPO4.12H2O
HO4P.2Na 141.958 g/mol
|
9 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 5 | Deionized water | 11.0 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 1.0, 10.0] | |
| 6 | 0.1 M HCl | 1 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 7 |
CoCl2.6H2O
2Cl.Co 129.839 g/mol
|
0.218 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 | NiCl3.6H2O | 0.118 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component | |
| 9 |
CuSO4.5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.156 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 10 |
CaCl2
Ca.2Cl 110.984 g/mol
|
7.8 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 11 |
FeCl3
Cl3Fe 162.204 g/mol
|
9.7 G_PER_L |
CHEBI:30808
iron trichloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 12 | CrCl3.6H2O | 0.105 G_PER_L | Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound | |
| 13 |
Fructose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:28645
beta-D-fructofuranose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
Solutions
Fructose solution
Dose: 10 G_PER_L
Trace element solution
Dose: 1 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- nitrogen_limited_mineral_salts_medium (CultureMech:009587)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.448941Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3115 |
| 2026-01-28T19:48:32.944399Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:54.528773+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:26.068560Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:07.090909Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009587 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.117079+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Nitrogen-limited mineral salts medium" to "nitrogen_limited_mineral_salts_medium" for consistent matching |
| 2026-05-16T09:04:27.673443+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:39.795414+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.593414+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:51.427477+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4c8c97cf0b1ce80c4e53225bf3db06f29fdeef325e2292f77a2f57e37bb2ad63 |