Overview
- External ontology
- TOGO:M3118 — Chemically defined medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M3118
Composition
flowchart LR
CultureMech_009589["chemically_defined_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009589 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_009589 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_009589 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Resazurin["Resazurin"]
CultureMech_009589 --> ing3_Resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Resazurin -.-> CHEBI_8806
ing4_Na2S_9H2O["Na2S.9H2O"]
CultureMech_009589 --> ing4_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing4_Na2S_9H2O -.-> CHEBI_76209
ing5_KCl["KCl"]
CultureMech_009589 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Na2SO4["Na2SO4"]
CultureMech_009589 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_CaCl2_6H2O["CaCl2.6H2O"]
CultureMech_009589 --> ing7_CaCl2_6H2O
CHEBI_91243["CHEBI:91243"]
ing7_CaCl2_6H2O -.-> CHEBI_91243
ing8_MgCl2_2H2O["MgCl2.2H2O"]
CultureMech_009589 --> ing8_MgCl2_2H2O
CHEBI_131394["CHEBI:131394"]
ing8_MgCl2_2H2O -.-> CHEBI_131394
ing9_3_M_HCl["3 M HCl"]
CultureMech_009589 --> ing9_3_M_HCl
ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009589 --> ing10_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing10_Carbon_dioxide_gas -.-> CHEBI_16526
ing11_Hydrogen_gas["Hydrogen gas"]
CultureMech_009589 --> ing11_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing11_Hydrogen_gas -.-> CHEBI_18276
ing12_Na2MoO4___H2O["Na2MoO4 × H2O"]
CultureMech_009589 --> ing12_Na2MoO4___H2O
CHEBI_75215["CHEBI:75215"]
ing12_Na2MoO4___H2O -.-> CHEBI_75215
ing13_H3BO3["H3BO3"]
CultureMech_009589 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_MnCl2___4H2O["MnCl2 × 4H2O"]
CultureMech_009589 --> ing14_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2___4H2O -.-> CHEBI_86368
ing15_CoCl2___6H2O["CoCl2 × 6H2O"]
CultureMech_009589 --> ing15_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing15_CoCl2___6H2O -.-> CHEBI_35696
ing16_ZnSO4___7H2O["ZnSO4 × 7H2O"]
CultureMech_009589 --> ing16_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4___7H2O -.-> CHEBI_32312
ing17_Na2WO4___2H2O["Na2WO4 × 2H2O"]
CultureMech_009589 --> ing17_Na2WO4___2H2O
CHEBI_63939["CHEBI:63939"]
ing17_Na2WO4___2H2O -.-> CHEBI_63939
ing18__NH4_2SO4FeSO4___6H2O["(NH4)2SO4FeSO4 × 6H2O"]
CultureMech_009589 --> ing18__NH4_2SO4FeSO4___6H2O
ing19__NH4_2SO4NiSO4___6H2O["(NH4)2SO4NiSO4 × 6H2O"]
CultureMech_009589 --> ing19__NH4_2SO4NiSO4___6H2O
ing20_Na2SeO4___5H2O["Na2SeO4 × 5H2O"]
CultureMech_009589 --> ing20_Na2SeO4___5H2O
ing21_CuCl2___6H2O["CuCl2 × 6H2O"]
CultureMech_009589 --> ing21_CuCl2___6H2O
CHEBI_49553["CHEBI:49553"]
ing21_CuCl2___6H2O -.-> CHEBI_49553
ing22_Biotin["Biotin"]
CultureMech_009589 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009589 --> ing23_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_009589 --> ing24_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing25_Folic_acid["Folic acid"]
CultureMech_009589 --> ing25_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing25_Folic_acid -.-> CHEBI_27470
ing26_Vitamin_B12["Vitamin B12"]
CultureMech_009589 --> ing26_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing26_Vitamin_B12 -.-> CHEBI_176843
ing27_Riboflavin["Riboflavin"]
CultureMech_009589 --> ing27_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing27_Riboflavin -.-> CHEBI_17015
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_009589 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Thiamine["Thiamine"]
CultureMech_009589 --> ing29_Thiamine
CHEBI_18385["CHEBI:18385"]
ing29_Thiamine -.-> CHEBI_18385
CultureMech_009589 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1002.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1000.0, 1.0] |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.33 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.33 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 5 |
Na2S.9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
2.8 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
CaCl2.6H2O
Ca.2Cl.6H2O 219.074 g/mol
|
0.33 G_PER_L |
CHEBI:91243
calcium chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
MgCl2.2H2O
2Cl.2H2O.Mg 131.241 g/mol
|
0.33 G_PER_L |
CHEBI:131394
magnesium dichloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 | 3 M HCl | variable VARIABLE | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 11 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 12 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 13 |
Na2MoO4 × H2O
MoO4.2Na 205.916 g/mol
|
24 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
6 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnCl2 × 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
99 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2 × 6H2O
2Cl.Co 129.839 g/mol
|
238 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
ZnSO4 × 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Na2WO4 × 2H2O
2H2O.2Na.O4W 329.846 g/mol
|
33 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 | (NH4)2SO4FeSO4 × 6H2O | 748 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 20 | (NH4)2SO4NiSO4 × 6H2O | 395 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 21 | Na2SeO4 × 5H2O | 94 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 22 |
CuCl2 × 6H2O
Cl2Cu 134.452 g/mol
|
238 G_PER_L |
CHEBI:49553
copper(II) chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Thiamine
C12H17N4OS 265.362 g/mol
|
5 G_PER_L |
CHEBI:18385
thiamine(1+)
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
5 G_PER_L |
CHEBI:7916
pantothenic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
Thioctic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:30314
(R)-lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
Solutions
Trace element solution
Dose: 1 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Vitamin solution
Dose: 1 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- chemically_defined_medium (CultureMech:009589)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.451733Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3118 |
| 2026-01-28T19:48:11.450999Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 3 M HCl; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:18.750344+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:46.477585Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:07.108473Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009589 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.133767+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Chemically defined medium" to "chemically_defined_medium" for consistent matching |
| 2026-05-16T09:03:27.230548+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:53.236315+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.452142+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:27.400920+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 35616d9fd9c73f82d04eefe4b45806f30c26a19edcc51833914bfdfdbfc3be55 |