Overview
- External ontology
- TOGO:M3126 — Mineral Medium For Chemolithotrophic Growth (H-3)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3126 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium81.pdf
Composition
flowchart LR
CultureMech_009596["mineral_medium_for_chemolithotrophic_growth_h_3"]
ing0_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009596 --> ing0_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing0_Carbon_dioxide_gas -.-> CHEBI_16526
ing1_Nitrogen_gas["Nitrogen gas"]
CultureMech_009596 --> ing1_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing1_Nitrogen_gas -.-> CHEBI_17997
ing2_Oxygen_gas["Oxygen gas"]
CultureMech_009596 --> ing2_Oxygen_gas
ing3_Hydrogen_gas["Hydrogen gas"]
CultureMech_009596 --> ing3_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing3_Hydrogen_gas -.-> CHEBI_18276
ing4_Distilled_water["Distilled water"]
CultureMech_009596 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
ing5_KH2PO4["KH2PO4"]
CultureMech_009596 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_009596 --> ing6_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing6_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009596 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009596 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_NH4Cl["NH4Cl"]
CultureMech_009596 --> ing9_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing9_NH4Cl -.-> CHEBI_31206
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_009596 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_NaVO3_x_H2O["NaVO3 x H2O"]
CultureMech_009596 --> ing11_NaVO3_x_H2O
CHEBI_132095["CHEBI:132095"]
ing11_NaVO3_x_H2O -.-> CHEBI_132095
ing12_Agar__if_necessary_["Agar (if necessary)"]
CultureMech_009596 --> ing12_Agar__if_necessary_
ing13_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_009596 --> ing13_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing13_Ferric_ammonium_citrate -.-> CHEBI_31604
ing14_Biotin["Biotin"]
CultureMech_009596 --> ing14_Biotin
CHEBI_15956["CHEBI:15956"]
ing14_Biotin -.-> CHEBI_15956
ing15_Ca_pantothenate["Ca-pantothenate"]
CultureMech_009596 --> ing15_Ca_pantothenate
CHEBI_29032["CHEBI:29032"]
ing15_Ca_pantothenate -.-> CHEBI_29032
ing16_Pyridoxine_HCl["Pyridoxine-HCl"]
CultureMech_009596 --> ing16_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing16_Pyridoxine_HCl -.-> CHEBI_30961
ing17_Folic_acid["Folic acid"]
CultureMech_009596 --> ing17_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing17_Folic_acid -.-> CHEBI_27470
ing18_Vitamin_B12["Vitamin B12"]
CultureMech_009596 --> ing18_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing18_Vitamin_B12 -.-> CHEBI_176843
ing19_Riboflavin["Riboflavin"]
CultureMech_009596 --> ing19_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing19_Riboflavin -.-> CHEBI_17015
ing20_Nicotinic_acid["Nicotinic acid"]
CultureMech_009596 --> ing20_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing20_Nicotinic_acid -.-> CHEBI_15940
ing21_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_009596 --> ing21_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing21_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009596 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_H3BO3["H3BO3"]
CultureMech_009596 --> ing23_H3BO3
CHEBI_33118["CHEBI:33118"]
ing23_H3BO3 -.-> CHEBI_33118
ing24_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_009596 --> ing24_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing24_CoCl2_x_6_H2O -.-> CHEBI_53503
ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009596 --> ing25_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
ing26_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_009596 --> ing26_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing26_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing27_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_009596 --> ing27_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing27_CuCl2_x_2_H2O -.-> CHEBI_86318
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 2 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 3 | Oxygen gas | variable VARIABLE | Properties: Gas | |
| 4 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: Gas |
| 5 |
Distilled water
H2O 18.015 g/mol
|
2085.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 50.0, 915.0, 20.0, 100.0, 1000.0] |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
2.9 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.01 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.034999999999999996 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.005, 0.03] |
| 12 |
NaVO3 x H2O
H2O.Na.O3V 139.946 g/mol
|
0.005 G_PER_L |
CHEBI:132095
sodium metavanadate hydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 | Agar (if necessary) | 20 G_PER_L | Role: Solidifying component | |
| 14 | Ferric ammonium citrate | 0.05 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 15 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.1 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Ca-pantothenate
C9H16NO5 218.229 g/mol
|
50 G_PER_L |
CHEBI:29032
(R)-pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
50 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 | Vitamin B12 | 1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
10 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
50 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
50 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Solution A
Dose: 50 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 920 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Solution C
Dose: 20 G_PER_L
See mediadive_5312_Solution_C.yaml for composition
Standard vitamin solution
Dose: 5 G_PER_L
See mediadive_864_Standard_vitamin_solution.yaml for composition
Trace element sol. SL-6
Dose: 5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M3126_Mineral_Medium_For_Chemolithotrophic_Growth_H-3 (CultureMech:009596)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.472056Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3126 |
| 2026-01-28T19:48:18.013229Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Oxygen gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:29.836954+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:08:58.728110Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:20:07.210121Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009596 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.236781+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mineral Medium For Chemolithotrophic Growth (H-3)" to "mineral_medium_for_chemolithotrophic_growth_h_3" for consistent matching |
| 2026-05-16T09:03:16.700643+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:39.972330+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:33.953676+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:29.331749+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.151479+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:14.589046+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b223b217c186699d0efb7749df2afc9316e44b8dfb85a136676ccba8263ce8d2 |