Overview
- External ontology
- TOGO:M3131 — Dsmz medium 854
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M3131
Composition
flowchart LR
CultureMech_009602["dsmz_medium_854"]
ing0_Distilled_water["Distilled water"]
CultureMech_009602 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009602 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_009602 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009602 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009602 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_009602 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_Resazurin["Resazurin"]
CultureMech_009602 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_009602 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_Na2S_9H2O["Na2S·9H2O"]
CultureMech_009602 --> ing8_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_9H2O -.-> CHEBI_76209
ing9_KCl["KCl"]
CultureMech_009602 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10_Sulfur_powder["Sulfur powder"]
CultureMech_009602 --> ing10_Sulfur_powder
CHEBI_33403["CHEBI:33403"]
ing10_Sulfur_powder -.-> CHEBI_33403
ing11_MOPS_buffer["MOPS buffer"]
CultureMech_009602 --> ing11_MOPS_buffer
CHEBI_44115["CHEBI:44115"]
ing11_MOPS_buffer -.-> CHEBI_44115
ing12_N2["N2"]
CultureMech_009602 --> ing12_N2
CHEBI_17997["CHEBI:17997"]
ing12_N2 -.-> CHEBI_17997
ing13_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009602 --> ing13_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing13_Carbon_dioxide_gas -.-> CHEBI_16526
ing14_Hydrogen_gas["Hydrogen gas"]
CultureMech_009602 --> ing14_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing14_Hydrogen_gas -.-> CHEBI_18276
ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009602 --> ing15_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009602 --> ing16_CaCl2_x_2_H2O
ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009602 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_H3BO3["H3BO3"]
CultureMech_009602 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_009602 --> ing19_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing19_FeSO4_x_7_H2O -.-> CHEBI_75836
ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009602 --> ing20_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_009602 --> ing21_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing22_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_009602 --> ing22_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing22_CuSO4_x_5_H2O -.-> CHEBI_31440
ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_009602 --> ing23_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing24_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_009602 --> ing24_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing24_CoSO4_x_7_H2O -.-> CHEBI_91244
ing25_AIK_SO4_2_x_12_H2O["AIK(SO4)2 x 12 H2O"]
CultureMech_009602 --> ing25_AIK_SO4_2_x_12_H2O
ing26_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009602 --> ing26_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing26_Nitrilotriacetic_acid -.-> CHEBI_44557
ing27_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_009602 --> ing27_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing27_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing28_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_009602 --> ing28_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing28_MnSO4_x_H2O -.-> CHEBI_86364
ing29_Biotin["Biotin"]
CultureMech_009602 --> ing29_Biotin
CHEBI_15956["CHEBI:15956"]
ing29_Biotin -.-> CHEBI_15956
CultureMech_009602 --> more["...8 more"]
Ingredients (38)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1002.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1000.0, 1.0] |
| 2 | Yeast extract | 200 MG_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
427.8 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Unit conflict noted: both MILLIMOLAR and G_PER_L present; MW=58.44 for conversion] |
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
2.2 MILLIMOLAR |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2.4 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
6.2 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 MG_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1.6 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
0.05 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
KCl
Cl.K 74.551 g/mol
|
4.4 MILLIMOLAR |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 | Sulfur powder | 10 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
MOPS buffer
C7H15NO4S 209.267 g/mol
|
14.3 MILLIMOLAR |
CHEBI:44115
3-(N-morpholino)propanesulfonic acid
|
Role: Buffer; Properties: Organic compound, Defined component, Solution |
| 13 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 14 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 15 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 16 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.3 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 | AIK(SO4)2 x 12 H2O | 0.02 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 27 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 28 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.4 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
Biotin
C10H16N2O3S 244.316 g/mol
|
20 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
4-aminobenzoic acid
C7H7NO2 137.138 g/mol
|
50 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
Folic acid
C19H19N7O6 441.404 g/mol
|
20 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
50 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 34 |
Pyridoxamine dihydrochloride
C8H12N2O2.2HCl 241.118 g/mol
|
100 G_PER_L |
CHEBI:131532
pyridoxamine dihydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 35 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
50 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 36 | dl-Ca-pantothenate | 50 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 37 |
Thiamine dihydrochloride
C12H17N4OS.Cl.HCl 337.276 g/mol
|
50 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Growth factor |
| 38 |
Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
1 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
Role: Vitamin |
Solutions
Trace element solution
Dose: 10 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Wolfe’s vitamin solution
Dose: 1 G_PER_L
KOH solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- dsmz_medium_854 (CultureMech:009602)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:57.490639Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3131 |
| 2026-01-28T19:48:19.202820Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: KOH solution |
| 2026-03-09T08:04:31.791633+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:00.957923Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s); WARNING: Duplicate 'NaCl' with different units: {'MILLIMOLAR', 'G_PER_L'} |
| 2026-03-13T04:23:43.749691Z | unit-conflict-resolver-v1.0 | Resolved unit conflicts | Documented molar unit conflict for NaCl |
| 2026-03-13T06:20:07.311811Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009602 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.339679+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Dsmz medium 854" to "dsmz_medium_854" for consistent matching |
| 2026-05-16T09:03:31.161572+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:59.133450+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:37.985787+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:42.798816+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:56.559643+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:33.054469+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d426b0832bcac579980abd607b7e7680da9de58846fb4506b197f81b03ad01fb |