Overview
- External ontology
- TOGO:M3136 — JCM medium 242
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M3136
Composition
flowchart LR
CultureMech_009605["jcm_medium_242"]
ing0_Distilled_water["Distilled water"]
CultureMech_009605 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009605 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_009605 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009605 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009605 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_009605 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_009605 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_Na2S_9H2O["Na2S・9H2O"]
CultureMech_009605 --> ing7_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_9H2O -.-> CHEBI_76209
ing8_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_009605 --> ing8_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_7H2O -.-> CHEBI_75836
ing9_NaHCO3["NaHCO3"]
CultureMech_009605 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10__NH4_2SO4["(NH4)2SO4"]
CultureMech_009605 --> ing10__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing10__NH4_2SO4 -.-> CHEBI_62946
ing11_Sodium_acetate["Sodium acetate"]
CultureMech_009605 --> ing11_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing11_Sodium_acetate -.-> CHEBI_32954
ing12_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009605 --> ing12_Yeast_extract__BD_Difco_
ing13_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_009605 --> ing13_Trypticase_peptone__BD_BBL_
ing14_Sodium_formate["Sodium formate"]
CultureMech_009605 --> ing14_Sodium_formate
CHEBI_62965["CHEBI:62965"]
ing14_Sodium_formate -.-> CHEBI_62965
ing15_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_009605 --> ing15_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing15_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing16_Trace_Mineral_Supplement__ATCC_["Trace Mineral Supplement (ATCC)"]
CultureMech_009605 --> ing16_Trace_Mineral_Supplement__ATCC_
ing17_Vitamin_supplement__ATCC_["Vitamin supplement (ATCC)"]
CultureMech_009605 --> ing17_Vitamin_supplement__ATCC_
ing18_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009605 --> ing18_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing18_Carbon_dioxide_gas -.-> CHEBI_16526
ing19_N2["N2"]
CultureMech_009605 --> ing19_N2
CHEBI_17997["CHEBI:17997"]
ing19_N2 -.-> CHEBI_17997
ing20_Hydrogen_gas["Hydrogen gas"]
CultureMech_009605 --> ing20_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing20_Hydrogen_gas -.-> CHEBI_18276
ing21_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009605 --> ing21_MgSO4___7H2O
ing21_MgSO4___7H2O -.-> CHEBI_31795
ing22_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009605 --> ing22_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4___2H2O -.-> CHEBI_75213
ing23_H3BO3["H3BO3"]
CultureMech_009605 --> ing23_H3BO3
CHEBI_33118["CHEBI:33118"]
ing23_H3BO3 -.-> CHEBI_33118
ing24_FeSO4___7H2O["FeSO4 . 7H2O"]
CultureMech_009605 --> ing24_FeSO4___7H2O
ing24_FeSO4___7H2O -.-> CHEBI_75836
ing25_NiCl2___6H2O["NiCl2 . 6H2O"]
CultureMech_009605 --> ing25_NiCl2___6H2O
CHEBI_34887["CHEBI:34887"]
ing25_NiCl2___6H2O -.-> CHEBI_34887
ing26_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_009605 --> ing26_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing26_ZnSO4___7H2O -.-> CHEBI_32312
ing27_CuSO4___5H2O["CuSO4 . 5H2O"]
CultureMech_009605 --> ing27_CuSO4___5H2O
CHEBI_31440["CHEBI:31440"]
ing27_CuSO4___5H2O -.-> CHEBI_31440
ing28_Na2WO4___2H2O["Na2WO4 . 2H2O"]
CultureMech_009605 --> ing28_Na2WO4___2H2O
CHEBI_63939["CHEBI:63939"]
ing28_Na2WO4___2H2O -.-> CHEBI_63939
ing29_CaCl2__anhydrous_["CaCl2 (anhydrous)"]
CultureMech_009605 --> ing29_CaCl2__anhydrous_
CultureMech_009605 --> more["...16 more"]
Ingredients (46)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
3.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.13 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
1.6 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.6, 1.0] |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
8 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
2.5 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 13 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 14 | Trypticase peptone (BD-BBL) | 2 G_PER_L | Properties: Undefined component | |
| 15 |
Sodium formate
CHO2.Na 68.007 g/mol
|
2.5 G_PER_L |
CHEBI:62965
sodium formate
|
Properties: Inorganic compound, Simple component, Defined component |
| 16 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 17 | Trace Mineral Supplement (ATCC) | 10 G_PER_L | ||
| 18 | Vitamin supplement (ATCC) | 10 G_PER_L | ||
| 19 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 20 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 21 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 22 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
NiCl2 . 6H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
CuSO4 . 5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
Na2WO4 . 2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.01 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 | CaCl2 (anhydrous) | 0.1 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 31 |
MnSO4 . H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 32 | AlK(SO4)2 (anhydrous) | 0.01 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 33 | Na2SeO3 (anhydrous) | 0.001 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 34 |
Co(NO3)2 . 6H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.1 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 35 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
Properties: Defined component, Simple component, Organic compound |
| 36 |
Monopotassium phosphate
H2O4P.K 136.084 g/mol
|
900 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 37 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 38 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 39 |
Calcium Pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 40 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 41 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 42 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 43 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 44 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 45 |
Thiamine
C12H17N4OS 265.362 g/mol
|
5 G_PER_L |
CHEBI:18385
thiamine(1+)
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 46 |
Thioctic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:30314
(R)-lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- jcm_medium_242 (CultureMech:009605)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.504478Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3136 |
| 2026-01-28T19:48:14.638187Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-13T03:08:52.706751Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:07.376707Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009605 |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.404889+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM medium 242" to "jcm_medium_242" for consistent matching |
| 2026-05-16T09:03:38.068464+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:09.759277+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:49.663648+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 2 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:00.169015+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:38.897043+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 59c5f39a2d7fd0abc03627c1f083b2f9c27e692adcc9d2c50dbe41adc270cebe |