Overview
- External ontology
- TOGO:M313 — Alkaline Agar
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M313 Original source: JCM - JCM_M318 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=318
Composition
flowchart LR
CultureMech_009607["alkaline_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_009607 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_009607 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_H2O["MgSO4・H2O"]
CultureMech_009607 --> ing2_MgSO4_H2O
CHEBI_32599["CHEBI:32599"]
ing2_MgSO4_H2O -.-> CHEBI_32599
ing3_Glucose["Glucose"]
CultureMech_009607 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Agar["Agar"]
CultureMech_009607 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009607 --> ing5_Yeast_extract__BD_Difco_
ing6_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
CultureMech_009607 --> ing6_Bacto_peptone__BD_Difco_
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
700 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
MgSO4・H2O
Mg.O4S 120.368 g/mol
|
0.2 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 5 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 6 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 7 | Bacto peptone (BD-Difco) | 5 G_PER_L | Properties: Undefined component |
Solutions
10% Na2CO3 solution
Dose: 100 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
20% NaCl solution
Dose: 200 G_PER_L
Role: Mineral source; Properties: Defined component, Solution, Inorganic compound
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M313_Alkaline_Agar (CultureMech:009607)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.836655Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M313 |
| 2026-01-28T19:48:06.819254Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:10.979351+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:07.407036Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009607 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.435266+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alkaline Agar" to "alkaline_agar" for consistent matching |
| 2026-05-16T09:03:16.842581+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:40.114427+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.206234+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:14.719812+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 96a1f522215ad47822e2075030e0a3da90c0ac0ad35f07153770ddb6c632c95d |