Overview
- External ontology
- TOGO:M3140 — Medium D
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3140
Composition
flowchart LR
CultureMech_009608["medium_d"]
ing0_MgSO4___7H2O["MgSO4 · 7H2O"]
CultureMech_009608 --> ing0_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4___7H2O -.-> CHEBI_31795
ing1_NaCl["NaCl"]
CultureMech_009608 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_NaNO3["NaNO3"]
CultureMech_009608 --> ing2_NaNO3
CHEBI_63005["CHEBI:63005"]
ing2_NaNO3 -.-> CHEBI_63005
ing3_NTA__sodium_nitrilotriacetate_["NTA (sodium nitrilotriacetate)"]
CultureMech_009608 --> ing3_NTA__sodium_nitrilotriacetate_
ing4_KNO3["KNO3"]
CultureMech_009608 --> ing4_KNO3
CHEBI_63043["CHEBI:63043"]
ing4_KNO3 -.-> CHEBI_63043
ing5_Na2NPO4["Na2NPO4"]
CultureMech_009608 --> ing5_Na2NPO4
ing6_CaSO4___2H2O["CaSO4 · 2H2O"]
CultureMech_009608 --> ing6_CaSO4___2H2O
CHEBI_32583["CHEBI:32583"]
ing6_CaSO4___2H2O -.-> CHEBI_32583
ing7_Double_distilled_water["Double-distilled water"]
CultureMech_009608 --> ing7_Double_distilled_water
ing8_Na2MoO4___2H2O["Na2MoO4 · 2H2O"]
CultureMech_009608 --> ing8_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing8_Na2MoO4___2H2O -.-> CHEBI_75213
ing9_H3BO3["H3BO3"]
CultureMech_009608 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_CoCl2___6H2O["CoCl2 · 6H2O"]
CultureMech_009608 --> ing10_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing10_CoCl2___6H2O -.-> CHEBI_35696
ing11_ZnSO4___7H2O["ZnSO4 · 7H2O"]
CultureMech_009608 --> ing11_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4___7H2O -.-> CHEBI_32312
ing12_CuSO4___5H2O["CuSO4 · 5H2O"]
CultureMech_009608 --> ing12_CuSO4___5H2O
CHEBI_31440["CHEBI:31440"]
ing12_CuSO4___5H2O -.-> CHEBI_31440
ing13_MnSO4___H2O["MnSO4 · H2O"]
CultureMech_009608 --> ing13_MnSO4___H2O
CHEBI_86364["CHEBI:86364"]
ing13_MnSO4___H2O -.-> CHEBI_86364
ing14_H2SO4__concentrated_["H2SO4 (concentrated)"]
CultureMech_009608 --> ing14_H2SO4__concentrated_
ing15_Distilled_water["Distilled water"]
CultureMech_009608 --> ing15_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing15_Distilled_water -.-> CHEBI_15377
ing16_FeCl3["FeCl3"]
CultureMech_009608 --> ing16_FeCl3
CHEBI_30808["CHEBI:30808"]
ing16_FeCl3 -.-> CHEBI_30808
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 · 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
0.008 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaNO3
NO3.Na 84.994 g/mol
|
0.7 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 4 | NTA (sodium nitrilotriacetate) | 0.1 G_PER_L | Role: Mineral source; Properties: Organic compound, Defined component, Simple component | |
| 5 |
KNO3
K.NO3 101.102 g/mol
|
0.1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 | Na2NPO4 | 0.11 G_PER_L | Role: Buffer; Properties: Inorganic compound, Defined component, Simple component | |
| 7 |
CaSO4 · 2H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.06 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 | Double-distilled water | 2.0 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 1.0] | |
| 9 |
Na2MoO4 · 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.025 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
0.5 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
CoCl2 · 6H2O
2Cl.Co 129.839 g/mol
|
0.045 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
ZnSO4 · 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.5 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CuSO4 · 5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.025 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
MnSO4 · H2O
H2O.Mn.O4S 169.016 g/mol
|
2.28 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 | H2SO4 (concentrated) | 0.5 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Solution | |
| 16 |
Distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
FeCl3
Cl3Fe 162.204 g/mol
|
0.29 G_PER_L |
CHEBI:30808
iron trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Micronutrient solution
Dose: 0.5 G_PER_L
See mediadive_6236_Micronutrient_solution.yaml for composition
FeCl3 solution (0.29 g/liter)
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- medium_d (CultureMech:009608)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.513648Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3140 |
| 2026-01-28T19:48:35.689101Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:59.398956+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:31.142202Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:07.415977Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009608 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.443528+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium D" to "medium_d" for consistent matching |
| 2026-05-16T09:04:19.780753+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:28.766891+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:20.815704+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:05.940453+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:42.927018+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 531d7e90be54b320093642b1929d4774c4eee0788c66681742f3290a2e46d06f |