Overview
- External ontology
- TOGO:M3142 — Chloroflexus medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3142 Original URL: https://www.atcc.org/-/media/product-assets/documents/microbial-media-formulations/9/2/0/atcc-medium-920.pdf?rev=17abd380bc714f0fbf0f8d4e7d9c200b
Composition
flowchart LR
CultureMech_009610["chloroflexus_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009610 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009610 --> ing1_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4___7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009610 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_009610 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_NH4Cl["NH4Cl"]
CultureMech_009610 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_NaNO3["NaNO3"]
CultureMech_009610 --> ing5_NaNO3
CHEBI_63005["CHEBI:63005"]
ing5_NaNO3 -.-> CHEBI_63005
ing6_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009610 --> ing6_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing6_Nitrilotriacetic_acid -.-> CHEBI_44557
ing7_KNO3["KNO3"]
CultureMech_009610 --> ing7_KNO3
CHEBI_63043["CHEBI:63043"]
ing7_KNO3 -.-> CHEBI_63043
ing8_Na2HPO4["Na2HPO4"]
CultureMech_009610 --> ing8_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing8_Na2HPO4 -.-> CHEBI_34683
ing9_CaSO4___2H2O["CaSO4 . 2H2O"]
CultureMech_009610 --> ing9_CaSO4___2H2O
CHEBI_32583["CHEBI:32583"]
ing9_CaSO4___2H2O -.-> CHEBI_32583
ing10_Sodium_sulfide["Sodium sulfide"]
CultureMech_009610 --> ing10_Sodium_sulfide
CHEBI_76208["CHEBI:76208"]
ing10_Sodium_sulfide -.-> CHEBI_76208
ing11_Glycyl_glycine["Glycyl-glycine"]
CultureMech_009610 --> ing11_Glycyl_glycine
CHEBI_17201["CHEBI:17201"]
ing11_Glycyl_glycine -.-> CHEBI_17201
ing12_FeCl3["FeCl3"]
CultureMech_009610 --> ing12_FeCl3
CHEBI_30808["CHEBI:30808"]
ing12_FeCl3 -.-> CHEBI_30808
ing13_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009610 --> ing13_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4___2H2O -.-> CHEBI_75213
ing14_H3BO3["H3BO3"]
CultureMech_009610 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_009610 --> ing15_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing15_CoCl2___6H2O -.-> CHEBI_35696
ing16_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_009610 --> ing16_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4___7H2O -.-> CHEBI_32312
ing17_CuSO4___2H2O["CuSO4 . 2H2O"]
CultureMech_009610 --> ing17_CuSO4___2H2O
CHEBI_23414["CHEBI:23414"]
ing17_CuSO4___2H2O -.-> CHEBI_23414
ing18_MnSO4___7H2O["MnSO4 . 7H2O"]
CultureMech_009610 --> ing18_MnSO4___7H2O
CHEBI_86360["CHEBI:86360"]
ing18_MnSO4___7H2O -.-> CHEBI_86360
ing19_H2SO4__concentrated_["H2SO4 (concentrated)"]
CultureMech_009610 --> ing19_H2SO4__concentrated_
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
3.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 1.0] |
| 2 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.008 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.2 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NaNO3
NO3.Na 84.994 g/mol
|
0.689 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.1 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 8 |
KNO3
K.NO3 101.102 g/mol
|
0.103 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.111 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CaSO4 . 2H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.06 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Sodium sulfide
2Na.S 78.047 g/mol
|
0.5 G_PER_L |
CHEBI:76208
sodium sulfide (anhydrous)
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Glycyl-glycine
C4H8N2O3 132.119 g/mol
|
0.5 G_PER_L |
CHEBI:17201
glycylglycine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 13 |
FeCl3
Cl3Fe 162.204 g/mol
|
0.2905 G_PER_L |
CHEBI:30808
iron trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.025 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.5 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
0.045 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.5 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuSO4 . 2H2O
Cu.O4S 159.609 g/mol
|
0.025 G_PER_L |
CHEBI:23414
copper(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
MnSO4 . 7H2O
Mn.O4S 151.001 g/mol
|
2.28 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 | H2SO4 (concentrated) | 0.5 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Solution |
Solutions
Micronutrient Solution
Dose: 1 G_PER_L
See mediadive_6236_Micronutrient_solution.yaml for composition
FeCl3 Solution
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M3142_Chloroflexus_medium (CultureMech:009610)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.519359Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3142 |
| 2026-01-28T19:48:23.521699Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:39.105511+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:08.943265Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:07.441264Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009610 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:15.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.468353+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Chloroflexus medium" to "chloroflexus_medium" for consistent matching |
| 2026-05-16T09:03:16.867879+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:40.140452+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:29.495688+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.215079+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:14.748946+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint cb6e8339e0072606ab613eb0e9f2f63dd7d0a00daa6ecd4a0fa9af27c0a3a2a3 |