modified_basal_medium

CultureMech:009619 bacterial COMPLEX LIQUID
Original name: Modified basal medium

Overview

External ontology
TOGO:M3160 — Modified basal medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M3160

Composition

flowchart LR
    CultureMech_009619["modified_basal_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009619 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_009619 --> ing1_Yeast_extract
    ing2_Resazurin["Resazurin"]
    CultureMech_009619 --> ing2_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing2_Resazurin -.-> CHEBI_8806
    ing3_Cysteine_HCl["Cysteine-HCl"]
    CultureMech_009619 --> ing3_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing3_Cysteine_HCl -.-> CHEBI_91247
    ing4_Vitamins["Vitamins"]
    CultureMech_009619 --> ing4_Vitamins
    ing5_CO["CO"]
    CultureMech_009619 --> ing5_CO
    ing6_N2["N2"]
    CultureMech_009619 --> ing6_N2
    CHEBI_17997["CHEBI:17997"]
    ing6_N2 -.-> CHEBI_17997
    ing7_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_009619 --> ing7_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing7_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing8_Sodium_tungstate["Sodium tungstate"]
    CultureMech_009619 --> ing8_Sodium_tungstate
    CHEBI_63940["CHEBI:63940"]
    ing8_Sodium_tungstate -.-> CHEBI_63940
    ing9_Cobalt_chloride["Cobalt chloride"]
    CultureMech_009619 --> ing9_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing9_Cobalt_chloride -.-> CHEBI_35696
    ing10_Cupric_chloride["Cupric chloride"]
    CultureMech_009619 --> ing10_Cupric_chloride
    CHEBI_49553["CHEBI:49553"]
    ing10_Cupric_chloride -.-> CHEBI_49553
    ing11_Sodium_selenate["Sodium selenate"]
    CultureMech_009619 --> ing11_Sodium_selenate
    CHEBI_77775["CHEBI:77775"]
    ing11_Sodium_selenate -.-> CHEBI_77775
    ing12_Nickel_chloride["Nickel chloride"]
    CultureMech_009619 --> ing12_Nickel_chloride
    CHEBI_34887["CHEBI:34887"]
    ing12_Nickel_chloride -.-> CHEBI_34887
    ing13_Sodium_molybdate["Sodium molybdate"]
    CultureMech_009619 --> ing13_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing13_Sodium_molybdate -.-> CHEBI_75215
    ing14_Manganese_sulfate["Manganese sulfate"]
    CultureMech_009619 --> ing14_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing14_Manganese_sulfate -.-> CHEBI_86364
    ing15_Zinc_sulfate["Zinc sulfate"]
    CultureMech_009619 --> ing15_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing15_Zinc_sulfate -.-> CHEBI_35176
    ing16_Ferrous_ammonium_sulfate["Ferrous ammonium sulfate"]
    CultureMech_009619 --> ing16_Ferrous_ammonium_sulfate
    ing17_Potassium_monophosphate["Potassium monophosphate"]
    CultureMech_009619 --> ing17_Potassium_monophosphate
    CHEBI_131527["CHEBI:131527"]
    ing17_Potassium_monophosphate -.-> CHEBI_131527
    ing18_Ammonium_chloride["Ammonium chloride"]
    CultureMech_009619 --> ing18_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing18_Ammonium_chloride -.-> CHEBI_31206
    ing19_Potassium_chloride["Potassium chloride"]
    CultureMech_009619 --> ing19_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing19_Potassium_chloride -.-> CHEBI_32588
    ing20_Calcium_chloride["Calcium chloride"]
    CultureMech_009619 --> ing20_Calcium_chloride
    CHEBI_86158["CHEBI:86158"]
    ing20_Calcium_chloride -.-> CHEBI_86158
    ing21_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_009619 --> ing21_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing21_Magnesium_sulfate -.-> CHEBI_32599
    ing22_Paraamino_benzoic_acid["Paraamino benzoic acid"]
    CultureMech_009619 --> ing22_Paraamino_benzoic_acid
    ing23_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_009619 --> ing23_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing23_Calcium_pantothenate -.-> CHEBI_31345
    ing24_Folic_acid["Folic acid"]
    CultureMech_009619 --> ing24_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing24_Folic_acid -.-> CHEBI_27470
    ing25_Vitamin_B12["Vitamin B12"]
    CultureMech_009619 --> ing25_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing25_Vitamin_B12 -.-> CHEBI_176843
    ing26_Riboflavin["Riboflavin"]
    CultureMech_009619 --> ing26_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing26_Riboflavin -.-> CHEBI_17015
    ing27_Nicotinic_acid["Nicotinic acid"]
    CultureMech_009619 --> ing27_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing27_Nicotinic_acid -.-> CHEBI_15940
    ing28_2_Mercaptoethanesulfonic_acid["2-Mercaptoethanesulfonic acid"]
    CultureMech_009619 --> ing28_2_Mercaptoethanesulfonic_acid
    CHEBI_17905["CHEBI:17905"]
    ing28_2_Mercaptoethanesulfonic_acid -.-> CHEBI_17905
    ing29_Thiamine["Thiamine"]
    CultureMech_009619 --> ing29_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing29_Thiamine -.-> CHEBI_18385
    CultureMech_009619 --> more["...3 more"]

Ingredients (33)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
4.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 1.0, 1.0, 1.0, 1.0]
2 Yeast extract 1 G_PER_L Role: Nutrient source; Properties: Undefined component
3 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
4 Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
0.6 G_PER_L CHEBI:91247
L-cysteine hydrochloride
 Properties: Defined component, Organic compound, Simple component
5 Vitamins 10 G_PER_L
6 CO variable VARIABLE Properties: Gas
7 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas
8 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
2 G_PER_L CHEBI:44557
nitrilotriacetic acid
 Role: Mineral source; Properties: Organic compound, Defined component, Simple component
9 Sodium tungstate
2Na.O4W 293.816 g/mol
20 G_PER_L CHEBI:63940
sodium tungstate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10 Cobalt chloride
2Cl.Co 129.839 g/mol
0.2 G_PER_L CHEBI:35696
cobalt dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11 Cupric chloride
Cl2Cu 134.452 g/mol
20 G_PER_L CHEBI:49553
copper(II) chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12 Sodium selenate
2Na.O4Se 188.936 g/mol
20 G_PER_L CHEBI:77775
sodium selenate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13 Nickel chloride
Cl2Ni 129.599 g/mol
20 G_PER_L CHEBI:34887
nickel dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14 Sodium molybdate
MoO4.2Na 205.916 g/mol
20 G_PER_L CHEBI:75215
sodium molybdate (anhydrous)
 Role: Mineral source; Properties: Organic compound, Defined component, Simple component
15 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
1 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
16 Zinc sulfate
O4S.Zn 161.453 g/mol
0.2 G_PER_L CHEBI:35176
zinc sulfate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
17 Ferrous ammonium sulfate 0.8 G_PER_L Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
18 Potassium monophosphate
HO4P.2K 174.174 g/mol
10 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
19 Ammonium chloride
Cl.H4N 53.492 g/mol
100 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
20 Potassium chloride
Cl.K 74.551 g/mol
10 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
21 Calcium chloride
Ca.2Cl.2H2O 147.014 g/mol
4 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
22 Magnesium sulfate
Mg.O4S 120.368 g/mol
20 G_PER_L CHEBI:32599
magnesium sulfate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
23 Paraamino benzoic acid 5 G_PER_L Role: Vitamin; Properties: Organic compound, Defined component, Simple component
24 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
5 G_PER_L CHEBI:31345
Calcium pantothenate
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
25 Folic acid
C19H19N7O6 441.404 g/mol
2 G_PER_L CHEBI:27470
folic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
26 Vitamin B12 5 G_PER_L CHEBI:176843
vitamin B12
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
27 Riboflavin
C17H20N4O6 376.369 g/mol
5 G_PER_L CHEBI:17015
riboflavin
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
28 Nicotinic acid
C6H5NO2 123.111 g/mol
5 G_PER_L CHEBI:15940
nicotinic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
29 2-Mercaptoethanesulfonic acid
C2H6O3S2 142.201 g/mol
2 G_PER_L CHEBI:17905
coenzyme M
 Role: Growth factor; Properties: Organic compound, Defined component, Simple component
30 Thiamine
C12H17N4OS 265.362 g/mol
5 G_PER_L CHEBI:18385
thiamine(1+)
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
31 Pyridoxine
C8H11NO3 169.18 g/mol
10 G_PER_L CHEBI:16709
pyridoxine
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
32 Thioctic acid
C8H14O2S2 206.332 g/mol
5 G_PER_L CHEBI:30314
(R)-lipoic acid
 Role: Growth factor; Properties: Organic compound, Defined component, Simple component
33 D-biotin
C10H16N2O3S 244.316 g/mol
2 G_PER_L CHEBI:15956
biotin
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component

Solutions

Mineral solution

Dose: 25 G_PER_L

See mediadive_5281_Mineral_solution.yaml for composition

Trace metal solution

Dose: 10 G_PER_L

See mediadive_6140_Trace_metal_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.545603Z togo-import Imported from TOGO Medium Source: TOGO, ID: M3160
2026-01-28T19:48:40.303435Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: CO; Added default concentration for ingredient: N2
2026-03-09T08:05:07.934555+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T03:09:39.653324Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:20:07.582323Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009619
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:26.605206+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified basal medium" to "modified_basal_medium" for consistent matching
2026-05-16T09:04:25.141488+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:41:36.309204+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:48.090225+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint a4b2448f8c08e231fac3a6eb666097eb4b0e2507809e5737004064cd77cf6ce2