Overview
- External ontology
- TOGO:M3163 — Standard PETC medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3163
Composition
flowchart LR
CultureMech_009621["standard_petc_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009621 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009621 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009621 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_009621 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009621 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_KH2PO4["KH2PO4"]
CultureMech_009621 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_NH4Cl["NH4Cl"]
CultureMech_009621 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_KCl["KCl"]
CultureMech_009621 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_Reducing_agent__100__["Reducing agent (100×)"]
CultureMech_009621 --> ing8_Reducing_agent__100__
ing9_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009621 --> ing9_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing9_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_009621 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009621 --> ing11_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_009621 --> ing12_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_009621 --> ing13_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
ing14_Nitrilotriacetic_acid__NTA_["Nitrilotriacetic acid (NTA)"]
CultureMech_009621 --> ing14_Nitrilotriacetic_acid__NTA_
CHEBI_44557["CHEBI:44557"]
ing14_Nitrilotriacetic_acid__NTA_ -.-> CHEBI_44557
ing15_Fe_SO4_2_NH4Cl_2_6H2O["Fe(SO4)2(NH4Cl)2⋅6H2O"]
CultureMech_009621 --> ing15_Fe_SO4_2_NH4Cl_2_6H2O
ing16_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_009621 --> ing16_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing16_MnSO4_x_H2O -.-> CHEBI_86364
ing17_Na2WO4["Na2WO4"]
CultureMech_009621 --> ing17_Na2WO4
CHEBI_63940["CHEBI:63940"]
ing17_Na2WO4 -.-> CHEBI_63940
ing18_Na2SeO4["Na2SeO4"]
CultureMech_009621 --> ing18_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing18_Na2SeO4 -.-> CHEBI_77775
ing19_Biotin["Biotin"]
CultureMech_009621 --> ing19_Biotin
CHEBI_15956["CHEBI:15956"]
ing19_Biotin -.-> CHEBI_15956
ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009621 --> ing20_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
ing21_Thiamin_HCl["Thiamin-HCl"]
CultureMech_009621 --> ing21_Thiamin_HCl
CHEBI_49105["CHEBI:49105"]
ing21_Thiamin_HCl -.-> CHEBI_49105
ing22_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_009621 --> ing22_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing22_Calcium_pantothenate -.-> CHEBI_31345
ing23_Pyridoxine_hydrochloride["Pyridoxine-hydrochloride"]
CultureMech_009621 --> ing23_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing23_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing24_Folic_acid["Folic acid"]
CultureMech_009621 --> ing24_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing24_Folic_acid -.-> CHEBI_27470
ing25_Cobalamin["Cobalamin"]
CultureMech_009621 --> ing25_Cobalamin
CHEBI_28911["CHEBI:28911"]
ing25_Cobalamin -.-> CHEBI_28911
ing26_Riboflavin["Riboflavin"]
CultureMech_009621 --> ing26_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing26_Riboflavin -.-> CHEBI_17015
ing27_Nicotinic_acid["Nicotinic acid"]
CultureMech_009621 --> ing27_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing27_Nicotinic_acid -.-> CHEBI_15940
ing28_Lipoic_acid["Lipoic acid"]
CultureMech_009621 --> ing28_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing28_Lipoic_acid -.-> CHEBI_16494
ing29_N2["N2"]
CultureMech_009621 --> ing29_N2
CHEBI_17997["CHEBI:17997"]
ing29_N2 -.-> CHEBI_17997
CultureMech_009621 --> more["...7 more"]
Ingredients (37)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
103.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 1.0, 1.0, 1.0, 100.0] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.8 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 | Reducing agent (100×) | 10 G_PER_L | ||
| 10 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.02 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.0002 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Nitrilotriacetic acid (NTA)
C6H9NO6 191.139 g/mol
|
2 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 16 | Fe(SO4)2(NH4Cl)2⋅6H2O | 0.8 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 17 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
1 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Na2WO4
2Na.O4W 293.816 g/mol
|
0.02 G_PER_L |
CHEBI:63940
sodium tungstate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.02 G_PER_L |
CHEBI:77775
sodium selenate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 |
Thiamin-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
Pyridoxine-hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 |
Cobalamin
C62H88CoN13O14P 1329.37 g/mol
|
0.1 G_PER_L |
CHEBI:28911
cob(III)alamin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 30 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 31 |
Fructose
C6H12O6 180.156 g/mol
|
25 G_PER_L |
CHEBI:28645
beta-D-fructofuranose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 32 | MES | 10 G_PER_L |
CHEBI:39010
MES
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 33 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 34 |
Na2S
2Na.S 78.047 g/mol
|
2.17 G_PER_L |
CHEBI:76208
sodium sulfide (anhydrous)
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 35 | Anaerobic water | 100 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component | |
| 36 |
Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
|
4 G_PER_L |
CHEBI:91247
L-cysteine hydrochloride
|
Properties: Defined component, Organic compound, Simple component |
| 37 |
NaOH
HO.Na 39.997 g/mol
|
0.9 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Inorganic compound, Simple component, Defined component |
Solutions
Resazurin-solution (0.025 vol%)
Dose: 4 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
Trace element solution (TE, 100×)
Dose: 10 G_PER_L
Wolfe’s vitamin solution (100×)
Dose: 10 G_PER_L
Fructose/MES solution (50×)
Dose: 20 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- standard_petc_medium (CultureMech:009621)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.553829Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3163 |
| 2026-01-28T19:48:08.732114Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: KOH; Added default concentration for ingredient: N2; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:14.067176+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:08:41.090211Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:20:07.621094Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009621 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.641501+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Standard PETC medium" to "standard_petc_medium" for consistent matching |
| 2026-05-16T09:04:32.443342+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:46.945786+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:50.594686+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:58:32.997988+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:11.812924+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:56.592056+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0ec9cd876307a4ea19e7403a49aa76b15f46b2ef4b8c9c0f4961d2b94e03101a |