Overview
- External ontology
- TOGO:M320 — <html>MJ/Anhox-NO<sub>3</sub> With Thiosulfate Medium</html>
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M320 Original source: JCM - JCM_M325 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=325
Composition
flowchart LR
CultureMech_009650["html_mj_anhox_no_sub_3_sub_with_thiosulfate_medium_html"]
ing0_Distilled_water["Distilled water"]
CultureMech_009650 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NH4Cl["NH4Cl"]
CultureMech_009650 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_NaHCO3["NaHCO3"]
CultureMech_009650 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_NaNO3["NaNO3"]
CultureMech_009650 --> ing3_NaNO3
CHEBI_63005["CHEBI:63005"]
ing3_NaNO3 -.-> CHEBI_63005
ing4_Na2SiO3["Na2SiO3"]
CultureMech_009650 --> ing4_Na2SiO3
ing5_Na2S2O3_5H2O["Na2S2O3・5H2O"]
CultureMech_009650 --> ing5_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing5_Na2S2O3_5H2O -.-> CHEBI_32150
ing6_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009650 --> ing6_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing6_Carbon_dioxide_gas -.-> CHEBI_16526
ing7_Hydrogen_gas["Hydrogen gas"]
CultureMech_009650 --> ing7_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing7_Hydrogen_gas -.-> CHEBI_18276
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
900 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.05 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
NaNO3
NO3.Na 84.994 g/mol
|
0.85 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 5 | Na2SiO3 | 0.6 G_PER_L | Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component | |
| 6 |
Na2S2O3・5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2.48 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Simple component |
| 7 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 8 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
Solutions
Trace vitamins (see Medium [M190])
Dose: 1 G_PER_L
Cross-reference to Medium M190
MJ(-N) synthetic seawater (see Medium [M260])
Dose: 100 G_PER_L
Cross-reference to Medium M260
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- html_mj_anhox_no_sub_3_sub_with_thiosulfate_medium_html (CultureMech:009650)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.849140Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M320 |
| 2026-01-28T19:48:31.044869Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-02-06T05:10:41.008083Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-09T08:04:51.653504+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:07.889402Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009650 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.895935+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "<html>MJ/Anhox-NO<sub>3</sub> With Thiosulfate Medium</html>" to "html_mj_anhox_no_sub_3_sub_with_thiosulfate_medium_html" for consistent matching |
| 2026-05-16T09:03:37.381300+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:08.820636+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.851976+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:37.959175+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 61993c7a16834169a0c11cbe3ce2c57f33a44b49d34e2135859873b84228bf6e |