formate_medium

CultureMech:009652 bacterial COMPLEX LIQUID
Original name: Formate medium

Overview

External ontology
TOGO:M3211 — Formate medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M3211

Composition

flowchart LR
    CultureMech_009652["formate_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009652 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_009652 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_009652 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_FeSO4["FeSO4"]
    CultureMech_009652 --> ing3_FeSO4
    CHEBI_75832["CHEBI:75832"]
    ing3_FeSO4 -.-> CHEBI_75832
    ing4_NaH2PO4["NaH2PO4"]
    CultureMech_009652 --> ing4_NaH2PO4
    CHEBI_37585["CHEBI:37585"]
    ing4_NaH2PO4 -.-> CHEBI_37585
    ing5_NaCOOH["NaCOOH"]
    CultureMech_009652 --> ing5_NaCOOH
    ing6_H2SO4["H2SO4"]
    CultureMech_009652 --> ing6_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing6_H2SO4 -.-> CHEBI_26836
    ing7_NaOH["NaOH"]
    CultureMech_009652 --> ing7_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing7_NaOH -.-> CHEBI_32145
    ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_009652 --> ing8_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_009652 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_009652 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_009652 --> ing11_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing12_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_009652 --> ing12_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing12_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing13_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_009652 --> ing13_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing13_MnSO4_x_H2O -.-> CHEBI_86364
    ing14_Ni2SO4___6H2O["Ni2SO4 โ‹… 6H2O"]
    CultureMech_009652 --> ing14_Ni2SO4___6H2O

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
2.0 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 1.0]
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.943 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.8 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
4 FeSO4
Fe.O4S 151.908 g/mol
0.016 G_PER_L CHEBI:75832
iron(2+) sulfate (anhydrous)
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5 NaH2PO4
H2O4P.Na 119.976 g/mol
4.68 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
6 NaCOOH 6.8 G_PER_L Properties: Defined component, Inorganic compound, Simple component
7 H2SO4
H2O4S 98.079 g/mol
variable VARIABLE CHEBI:26836
sulfuric acid
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
8 NaOH
HO.Na 39.997 g/mol
variable VARIABLE CHEBI:32145
sodium hydroxide
Properties: Defined component, Inorganic compound, Solution
9 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
1.8 G_PER_L CHEBI:75213
sodium molybdate dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.5 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
2.4 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.48 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
4.02 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
2.4 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
15 Ni2SO4 โ‹… 6H2O 1.5 G_PER_L Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component

Solutions

Trace element solution

Dose: 1 G_PER_L

See mediadive_6187_Trace_element_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.600322Z togo-import Imported from TOGO Medium Source: TOGO, ID: M3211
2026-01-28T19:48:23.182447Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: NaOH
2026-03-09T08:04:38.534355+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:09:08.328078Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:20:07.902786Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009652
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:14.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:26.908890+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Formate medium" to "formate_medium" for consistent matching
2026-05-16T09:03:35.115871+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:41:05.374640+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:46.715597+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4ยท7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:58.770790+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:35.560475+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 7d91ba4cb9511edad9c73a8329c1aa0d7e47dc853d4c41bc41869fbaff63adc5