Overview
- External ontology
- TOGO:M3216 — Liquid Fermentation Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3216
Composition
flowchart LR
CultureMech_009657["liquid_fermentation_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009657 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009657 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_009657 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_009657 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_Na2HPO4_12H2O["Na2HPO4·12H2O"]
CultureMech_009657 --> ing4_Na2HPO4_12H2O
CHEBI_34683["CHEBI:34683"]
ing4_Na2HPO4_12H2O -.-> CHEBI_34683
ing5_Fructose["Fructose"]
CultureMech_009657 --> ing5_Fructose
CHEBI_28645["CHEBI:28645"]
ing5_Fructose -.-> CHEBI_28645
ing6_35__HCl["35% HCl"]
CultureMech_009657 --> ing6_35__HCl
ing7_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_009657 --> ing7_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2MoO4_2H2O -.-> CHEBI_75213
ing8_H3BO3["H3BO3"]
CultureMech_009657 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_009657 --> ing9_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_7H2O -.-> CHEBI_75836
ing10_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_009657 --> ing10_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing10_ZnSO4_7H2O -.-> CHEBI_32312
ing11_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_009657 --> ing11_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing11_CuSO4_5H2O -.-> CHEBI_31440
ing12_MnSO4_5H2O["MnSO4·5H2O"]
CultureMech_009657 --> ing12_MnSO4_5H2O
CHEBI_131524["CHEBI:131524"]
ing12_MnSO4_5H2O -.-> CHEBI_131524
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Na2HPO4·12H2O
HO4P.2Na 141.958 g/mol
|
10 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Fructose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:28645
beta-D-fructofuranose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 7 | 35% HCl | 10 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 8 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.145 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
0.15 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
10 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
2.25 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
MnSO4·5H2O
5H2O.Mn.O4S 241.076 g/mol
|
0.5 G_PER_L |
CHEBI:131524
manganese(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace element solution
Dose: 1 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- liquid_fermentation_medium (CultureMech:009657)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.607083Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3216 |
| 2026-01-28T19:48:15.841109Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:26.121993+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:54.675333Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:07.932686Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009657 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:59.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.938210+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Liquid Fermentation Medium" to "liquid_fermentation_medium" for consistent matching |
| 2026-05-16T09:03:39.098932+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:11.231681+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:50.664753+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:00.570825+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:40.321523+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5a2eadd165b025168234206e1dca1b004f447733b51d6a5a73d941c8ed0e2ebd |