Overview
- External ontology
- TOGO:M3217 — Luria–Bertani (LB) + antibiotics
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3217 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009658["luria_bertani_lb_antibiotics"]
ing0_Ampicillin["Ampicillin"]
CultureMech_009658 --> ing0_Ampicillin
CHEBI_28971["CHEBI:28971"]
ing0_Ampicillin -.-> CHEBI_28971
ing1_Kanamycin["Kanamycin"]
CultureMech_009658 --> ing1_Kanamycin
ing2_Rifampicin["Rifampicin"]
CultureMech_009658 --> ing2_Rifampicin
CHEBI_28077["CHEBI:28077"]
ing2_Rifampicin -.-> CHEBI_28077
ing3_Tetracycline["Tetracycline"]
CultureMech_009658 --> ing3_Tetracycline
ing4_Gentamicin["Gentamicin"]
CultureMech_009658 --> ing4_Gentamicin
ing5_Tryptone["Tryptone"]
CultureMech_009658 --> ing5_Tryptone
ing6_Yeast_extract["Yeast extract"]
CultureMech_009658 --> ing6_Yeast_extract
ing7_Sodium_chloride["Sodium chloride"]
CultureMech_009658 --> ing7_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing7_Sodium_chloride -.-> CHEBI_26710
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Ampicillin
C16H19N3O4S 349.412 g/mol
|
100 G_PER_L |
CHEBI:28971
ampicillin
|
Role: Antimicrobial agent; Properties: Defined component, Organic compound, Simple component |
| 2 | Kanamycin | 50 G_PER_L | Role: Antimicrobial agent; Properties: Organic compound, Simple component, Defined component | |
| 3 |
Rifampicin
C43H58N4O12 822.953 g/mol
|
50 G_PER_L |
CHEBI:28077
rifampicin
|
Role: Antimicrobial agent; Properties: Simple component, Defined component, Organic compound |
| 4 | Tetracycline | 15 G_PER_L | Role: Antimicrobial agent; Properties: Simple component, Defined component | |
| 5 | Gentamicin | 50 G_PER_L | Role: Antimicrobial agent; Properties: Simple component, Defined component, Organic compound | |
| 6 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 7 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 8 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- luria_bertani_lb_antibiotics (CultureMech:009658)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.609023Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3217 |
| 2026-01-28T19:48:32.071791Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:07.938543Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009658 |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.944550+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Luria–Bertani (LB) + antibiotics" to "luria_bertani_lb_antibiotics" for consistent matching |
| 2026-06-05T07:41:11.489122+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:40.617264+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e22626294893098fe5a9dfc7babf6fac81db845182d09857c737f24991a4e9f1 |