Overview
- External ontology
- TOGO:M3220 — MM succinate (MMS)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3220
Composition
flowchart LR
CultureMech_009660["mm_succinate_mms"]
ing0_Distilled_water["Distilled water"]
CultureMech_009660 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009660 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_009660 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_009660 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_Na2HPO4["Na2HPO4"]
CultureMech_009660 --> ing4_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing4_Na2HPO4 -.-> CHEBI_34683
ing5_Succinate["Succinate"]
CultureMech_009660 --> ing5_Succinate
CHEBI_26806["CHEBI:26806"]
ing5_Succinate -.-> CHEBI_26806
ing6_Ampicillin["Ampicillin"]
CultureMech_009660 --> ing6_Ampicillin
CHEBI_28971["CHEBI:28971"]
ing6_Ampicillin -.-> CHEBI_28971
ing7_Kanamycin["Kanamycin"]
CultureMech_009660 --> ing7_Kanamycin
ing8_H3BO3["H3BO3"]
CultureMech_009660 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_FeSO4_7H2O["FeSO4.7H2O"]
CultureMech_009660 --> ing9_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_7H2O -.-> CHEBI_75836
ing10_ZnSO4_7H2O["ZnSO4.7H2O"]
CultureMech_009660 --> ing10_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing10_ZnSO4_7H2O -.-> CHEBI_32312
ing11_CuSO4_5H2O["CuSO4.5H2O"]
CultureMech_009660 --> ing11_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing11_CuSO4_5H2O -.-> CHEBI_31440
ing12_CoSO4_7H2O["CoSO4.7H2O"]
CultureMech_009660 --> ing12_CoSO4_7H2O
CHEBI_53470["CHEBI:53470"]
ing12_CoSO4_7H2O -.-> CHEBI_53470
ing13_CaCO3["CaCO3"]
CultureMech_009660 --> ing13_CaCO3
CHEBI_3311["CHEBI:3311"]
ing13_CaCO3 -.-> CHEBI_3311
ing14_MgSO4["MgSO4"]
CultureMech_009660 --> ing14_MgSO4
CHEBI_32599["CHEBI:32599"]
ing14_MgSO4 -.-> CHEBI_32599
ing15_MnSO4_7H2O["MnSO4.7H2O"]
CultureMech_009660 --> ing15_MnSO4_7H2O
CHEBI_86360["CHEBI:86360"]
ing15_MnSO4_7H2O -.-> CHEBI_86360
ing16_Concentrated_hydrochloric_acid["Concentrated hydrochloric acid"]
CultureMech_009660 --> ing16_Concentrated_hydrochloric_acid
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 | Succinate | 20 MILLIMOLAR |
CHEBI:26806
succinate
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 7 |
Ampicillin
C16H19N3O4S 349.412 g/mol
|
300 G_PER_L |
CHEBI:28971
ampicillin
|
Role: Antimicrobial agent; Properties: Organic compound, Defined component, Simple component |
| 8 | Kanamycin | 10 G_PER_L | Role: Antimicrobial agent; Properties: Organic compound, Defined component, Simple component | |
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
FeSO4.7H2O
Fe.7H2O.O4S 278.013 g/mol
|
4.5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
ZnSO4.7H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.44 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CuSO4.5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.25 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CoSO4.7H2O
Co.O4S 154.996 g/mol
|
0.28 G_PER_L |
CHEBI:53470
cobalt(2+) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CaCO3
CO3.Ca 100.086 g/mol
|
2 G_PER_L |
CHEBI:3311
calcium carbonate
|
Role: Protective agent, Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MgSO4
Mg.O4S 120.368 g/mol
|
10.75 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
MnSO4.7H2O
Mn.O4S 151.001 g/mol
|
1.12 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 | Concentrated hydrochloric acid | 51.3 G_PER_L | Role: Solvating media |
Solutions
Saturated CaCl2 solution
Dose: 10 G_PER_L
Role: Mineral source; Properties: Inorganic compound, Defined component, Solution
Saturated FeSO4 solution
Dose: 10 G_PER_L
Role: Mineral source; Properties: Inorganic compound, Defined component, Solution
Bauchop & Elsden solution
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mm_succinate_mms (CultureMech:009660)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.610080Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3220 |
| 2026-01-28T19:48:17.001033Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:28.108688+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:08:56.864437Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:07.953434Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009660 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.958844+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MM succinate (MMS)" to "mm_succinate_mms" for consistent matching |
| 2026-05-16T09:04:24.407066+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:35.291389+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:25.089933+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:08.235655+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:46.926581+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5ec5d14bdf40e59090c234552429dba35117a9cd813231c139519cee6e8b1192 |