9_k_medium

CultureMech:009675 bacterial COMPLEX LIQUID
Original name: 9 K medium

Overview

External ontology
TOGO:M3238 — 9 K medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M3238

Composition

flowchart LR
    CultureMech_009675["9_k_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009675 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_009675 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_K2HPO4["K2HPO4"]
    CultureMech_009675 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_KCl["KCl"]
    CultureMech_009675 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_009675 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_Ca_NO3_2["Ca(NO3)2"]
    CultureMech_009675 --> ing5_Ca_NO3_2
    CHEBI_64205["CHEBI:64205"]
    ing5_Ca_NO3_2 -.-> CHEBI_64205

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3 K2HPO4
HO4P.2K 174.174 g/mol
0.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
4 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
3 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Simple component, Defined component, Inorganic compound
6 Ca(NO3)2
Ca.2NO3 164.086 g/mol
0.01 G_PER_L CHEBI:64205
calcium nitrate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.628596Z togo-import Imported from TOGO Medium Source: TOGO, ID: M3238
2026-01-28T19:48:38.769697Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:08.033111Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009675
2026-03-17T06:30:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.037989+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "9 K medium" to "9_k_medium" for consistent matching
2026-06-05T07:40:15.976496+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.188015+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T06:57:12.645302+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:42.588786+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:34.246503+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 7b9c0139fb40ea46919010dc7e90eb25818c93c09c02cb406a65101d1a11135a