Overview
- External ontology
- TOGO:M3240 — Modified 9K medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M3240
Composition
flowchart LR
CultureMech_009678["modified_9k_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009678 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009678 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_009678 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_009678 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_Fe2_SO4_3["Fe2(SO4)3"]
CultureMech_009678 --> ing4_Fe2_SO4_3
ing5_Concentrated_H2SO4["Concentrated H2SO4"]
CultureMech_009678 --> ing5_Concentrated_H2SO4
ing6_N2_gas["N2 gas"]
CultureMech_009678 --> ing6_N2_gas
ing7_Hydrogen_gas["Hydrogen gas"]
CultureMech_009678 --> ing7_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing7_Hydrogen_gas -.-> CHEBI_18276
ing8_H3BO3["H3BO3"]
CultureMech_009678 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_009678 --> ing9_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_7H2O -.-> CHEBI_32312
ing10_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_009678 --> ing10_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing10_CuCl2_2H2O -.-> CHEBI_86318
ing11_MnCl2_2H2O["MnCl2·2H2O"]
CultureMech_009678 --> ing11_MnCl2_2H2O
CHEBI_131395["CHEBI:131395"]
ing11_MnCl2_2H2O -.-> CHEBI_131395
ing12_Na2MoO4["Na2MoO4"]
CultureMech_009678 --> ing12_Na2MoO4
CHEBI_75215["CHEBI:75215"]
ing12_Na2MoO4 -.-> CHEBI_75215
ing13_NaVO3["NaVO3"]
CultureMech_009678 --> ing13_NaVO3
CHEBI_75221["CHEBI:75221"]
ing13_NaVO3 -.-> CHEBI_75221
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.4 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.4 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 5 | Fe2(SO4)3 | 2.5 G_PER_L | Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component | |
| 6 | Concentrated H2SO4 | variable VARIABLE | Role: Mineral source; Properties: Defined component, Inorganic compound, Solution | |
| 7 | N2 gas | variable VARIABLE | Properties: ガス | |
| 8 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
30 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
68 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 11 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
66 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 12 |
MnCl2·2H2O
Cl2Mn.2H2O 161.874 g/mol
|
62 G_PER_L |
CHEBI:131395
manganese(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 13 |
Na2MoO4
MoO4.2Na 205.916 g/mol
|
10 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
Role: Mineral source; Properties: Simple component, Defined component, Organic compound |
| 14 |
NaVO3
Na.O3V 121.931 g/mol
|
30 G_PER_L |
CHEBI:75221
sodium metavanadate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
Solutions
Trace element solution
Dose: 1 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_9k_medium (CultureMech:009678)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.630688Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3240 |
| 2026-01-28T19:48:16.826899Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Concentrated H2SO4; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:27.796658+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:56.552209Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:08.049745Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009678 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.054621+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified 9K medium" to "modified_9k_medium" for consistent matching |
| 2026-05-16T09:04:24.968836+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:36.084565+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:25.624509+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:08.481823+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:47.872253+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7d68362a8aa2a71c38f31cfd4b02a4573f7923cfcedef8ef01e3a060cff814f6 |