Overview
- External ontology
- TOGO:M3265 — Fe- and S-free basal medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3265
Composition
flowchart LR
CultureMech_009688["fe_and_s_free_basal_medium"]
ing0_NaCl["NaCl"]
CultureMech_009688 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_NH4Cl["NH4Cl"]
CultureMech_009688 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_CaCl2___2H2O["CaCl2 · 2H2O"]
CultureMech_009688 --> ing2_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2___2H2O -.-> CHEBI_86158
ing3_MgCl2___6H2O["MgCl2 · 6H2O"]
CultureMech_009688 --> ing3_MgCl2___6H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2___6H2O -.-> CHEBI_86345
ing4_KCl["KCl"]
CultureMech_009688 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaHCO3["NaHCO3"]
CultureMech_009688 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_MQ_H2O["MQ H2O"]
CultureMech_009688 --> ing6_MQ_H2O
ing7_2_M_HCl["2 M HCl"]
CultureMech_009688 --> ing7_2_M_HCl
ing8_1_M_NaOH["1 M NaOH"]
CultureMech_009688 --> ing8_1_M_NaOH
ing9_N2["N2"]
CultureMech_009688 --> ing9_N2
CHEBI_17997["CHEBI:17997"]
ing9_N2 -.-> CHEBI_17997
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
21.98 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
CaCl2 · 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
MgCl2 · 6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 7 | MQ H2O | 1 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component | |
| 8 | 2 M HCl | variable VARIABLE | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 9 | 1 M NaOH | variable VARIABLE | Properties: Defined component, Inorganic compound, Solution | |
| 10 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- fe_and_s_free_basal_medium (CultureMech:009688)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.646071Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3265 |
| 2026-01-28T19:48:20.248809Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 2 M HCl; Added default concentration for ingredient: 1 M NaOH; Added default concentration for ingredient: N2 |
| 2026-03-13T06:20:08.146625Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009688 |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.137027+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Fe- and S-free basal medium" to "fe_and_s_free_basal_medium" for consistent matching |
| 2026-05-16T09:03:31.680570+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:59.866590+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.776056+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:33.874574+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 37adf9b9803d247d5fd5af925cee4f269ae1af460a67ab66fc975ada7cc8a90a |