Overview
- External ontology
- TOGO:M3274 — Pfennig Ignisphaera Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3274 Original source: JCM - JCM_M1420 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1420
Composition
flowchart LR
CultureMech_009692["pfennig_ignisphaera_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009692 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009692 --> ing1_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_2H2O -.-> CHEBI_86158
ing2_KH2PO4["KH2PO4"]
CultureMech_009692 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_009692 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_Resazurin["Resazurin"]
CultureMech_009692 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_009692 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6_KCl["KCl"]
CultureMech_009692 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_Beef_extract["Beef extract"]
CultureMech_009692 --> ing7_Beef_extract
ing8_Yeast_extract__Oxoid_["Yeast extract (Oxoid)"]
CultureMech_009692 --> ing8_Yeast_extract__Oxoid_
ing9_N2["N2"]
CultureMech_009692 --> ing9_N2
CHEBI_17997["CHEBI:17997"]
ing9_N2 -.-> CHEBI_17997
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.33 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.33 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.33 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 6 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.33 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 | Beef extract | 0.5 G_PER_L | Properties: Undefined component | |
| 9 | Yeast extract (Oxoid) | 0.2 G_PER_L | Properties: Undefined component, Complex component | |
| 10 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Solutions
Trace element solution (see Medium [M940])
Dose: 1 G_PER_L
Cross-reference to Medium M940
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
5% Na2S•9H2O solution
Dose: 6 G_PER_L
Role: Reducing agent; Properties: Solution, Inorganic compound, Defined component
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pfennig_ignisphaera_medium (CultureMech:009692)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.719030Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M3274 |
| 2026-01-28T19:48:38.244325Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:05:03.984168+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:20:08.182843Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009692 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.171921+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Pfennig Ignisphaera Medium" to "pfennig_ignisphaera_medium" for consistent matching |
| 2026-05-16T09:04:28.856240+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:41.517028+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.099879+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:32.266629+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8130f8929176fc32fb55811f9ff5df231ac0e4dc93e97913ee5890ae4e646b45 |