mnyp_medium

CultureMech:009700 bacterial COMPLEX LIQUID
Original name: MNYP medium

Overview

External ontology
TOGO:M3286 — MNYP medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M3286 Original source: NBRC - NBRC_M1647-1 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1647

Composition

flowchart LR
    CultureMech_009700["mnyp_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009700 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Sodium_acetate["Sodium acetate"]
    CultureMech_009700 --> ing1_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing1_Sodium_acetate -.-> CHEBI_32954
    ing2_Mannitol["Mannitol"]
    CultureMech_009700 --> ing2_Mannitol
    CHEBI_29864["CHEBI:29864"]
    ing2_Mannitol -.-> CHEBI_29864
    ing3_Ager["Ager"]
    CultureMech_009700 --> ing3_Ager
    ing4_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
    CultureMech_009700 --> ing4_Bacto_Yeast_Extract__Difco_
    ing5_Tween_80["Tween 80"]
    CultureMech_009700 --> ing5_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing5_Tween_80 -.-> CHEBI_53426
    ing6_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_009700 --> ing6_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_7H2O -.-> CHEBI_31795
    ing7_NaCl["NaCl"]
    CultureMech_009700 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_009700 --> ing8_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_7H2O -.-> CHEBI_75836
    ing9_MnSO4_4H2O["MnSO4·4H2O"]
    CultureMech_009700 --> ing9_MnSO4_4H2O
    CHEBI_86358["CHEBI:86358"]
    ing9_MnSO4_4H2O -.-> CHEBI_86358
    ing10_HCl["HCl"]
    CultureMech_009700 --> ing10_HCl
    CHEBI_17883["CHEBI:17883"]
    ing10_HCl -.-> CHEBI_17883

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
3.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Merged 3 duplicates: 1.0, 1.0, 1.0]
2 Sodium acetate
C2H3O2.Na 82.034 g/mol
2 G_PER_L CHEBI:32954
sodium acetate
 Role: Buffer; Properties: Defined component, Simple component, Organic compound
3 Mannitol
C6H14O6 182.172 g/mol
10 G_PER_L CHEBI:29864
mannitol
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
4 Ager 15 G_PER_L Role: Solidifying component
5 Bacto Yeast Extract (Difco) 10 G_PER_L Properties: Undefined component
6 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
50 G_PER_L CHEBI:53426
polysorbate 80
 Role: Surfactant; Properties: Defined component, Organic compound, Simple component
7 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
40 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
8 NaCl
Cl.Na 58.443 g/mol
2 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
9 FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
10 MnSO4·4H2O
4H2O.Mn.O4S 223.061 g/mol
2 G_PER_L CHEBI:86358
manganese(II) sulfate tetrahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
11 HCl
HCl 36.461 g/mol
variable VARIABLE CHEBI:17883
hydrogen chloride
 Role: Solvating media; Properties: Defined component, Inorganic compound, Solution

Solutions

Hipolypepton*

Dose: 5 G_PER_L

Properties: Undefined component

Tween 80 solution**

Dose: 10 G_PER_L

Salts solution***

Dose: 5 G_PER_L

See mediadive_4429_Salts_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:59.260506Z togo-import Imported from TOGO Medium Source: NBRC, ID: M3286
2026-01-28T19:48:39.963101Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: HCl
2026-03-09T08:05:07.289485+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T03:09:39.010385Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:20:08.237268Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009700
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.224631+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MNYP medium" to "mnyp_medium" for consistent matching
2026-05-16T09:04:24.794048+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:41:35.859656+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:25.446200+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-08-06T03:05:47.617750+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 27c614d7991231d0ee9d79f734de2cbf414418395037b7a05958aa421aa492cf