modified_pyg_medium

CultureMech:009703 bacterial COMPLEX LIQUID
Original name: modified PYG Medium

Overview

External ontology
TOGO:M3290 — modified PYG Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M3290 Original source: NBRC - NBRC_M1660 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1660

Composition

flowchart LR
    CultureMech_009703["modified_pyg_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009703 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_K2HPO4["K2HPO4"]
    CultureMech_009703 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_Resazurin["Resazurin"]
    CultureMech_009703 --> ing2_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing2_Resazurin -.-> CHEBI_8806
    ing3_Tween_80["Tween 80"]
    CultureMech_009703 --> ing3_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing3_Tween_80 -.-> CHEBI_53426
    ing4_Glucose["Glucose"]
    CultureMech_009703 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
    CultureMech_009703 --> ing5_Bacto_Yeast_Extract__Difco_
    ing6_Cysteine_HCl["Cysteine-HCl"]
    CultureMech_009703 --> ing6_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing6_Cysteine_HCl -.-> CHEBI_91247
    ing7_Beef_extract["Beef extract"]
    CultureMech_009703 --> ing7_Beef_extract
    ing8_Tripticase_peptone["Tripticase peptone"]
    CultureMech_009703 --> ing8_Tripticase_peptone
    ing9_Peptone["Peptone"]
    CultureMech_009703 --> ing9_Peptone
    ing10_N2["N2"]
    CultureMech_009703 --> ing10_N2
    CHEBI_17997["CHEBI:17997"]
    ing10_N2 -.-> CHEBI_17997
    ing11_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_009703 --> ing11_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing11_MgSO4_7H2O -.-> CHEBI_31795
    ing12_NaCl["NaCl"]
    CultureMech_009703 --> ing12_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing12_NaCl -.-> CHEBI_26710
    ing13_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_009703 --> ing13_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing13_CaCl2_2H2O -.-> CHEBI_86158
    ing14_KH2PO4["KH2PO4"]
    CultureMech_009703 --> ing14_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing14_KH2PO4 -.-> CHEBI_63036
    ing15_NaHCO3["NaHCO3"]
    CultureMech_009703 --> ing15_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing15_NaHCO3 -.-> CHEBI_32139
    ing16_Ethanol["Ethanol"]
    CultureMech_009703 --> ing16_Ethanol
    CHEBI_16236["CHEBI:16236"]
    ing16_Ethanol -.-> CHEBI_16236
    ing17_vitamin_K1["vitamin K1"]
    CultureMech_009703 --> ing17_vitamin_K1
    CHEBI_18067["CHEBI:18067"]
    ing17_vitamin_K1 -.-> CHEBI_18067
    ing18_NaOH["NaOH"]
    CultureMech_009703 --> ing18_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing18_NaOH -.-> CHEBI_32145
    ing19_Hemin["Hemin"]
    CultureMech_009703 --> ing19_Hemin
    CHEBI_50385["CHEBI:50385"]
    ing19_Hemin -.-> CHEBI_50385

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1051.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 950.0, 1.0, 100.0]
2 K2HPO4
HO4P.2K 174.174 g/mol
3.0 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 2.0, 1.0]
3 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
4 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
1 G_PER_L CHEBI:53426
polysorbate 80
 Role: Surfactant; Properties: Organic compound, Defined component, Simple component
5 Glucose
C6H12O6 180.156 g/mol
5 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Organic compound, Defined component, Simple component
6 Bacto Yeast Extract (Difco) 10 G_PER_L Properties: Undefined component
7 Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
0.5 G_PER_L CHEBI:91247
L-cysteine hydrochloride
 Properties: Defined component, Simple component, Organic compound
8 Beef extract 5 G_PER_L Properties: Undefined component
9 Tripticase peptone 5 G_PER_L Properties: Undefined component
10 Peptone 5 G_PER_L
11 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas
12 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13 NaCl
Cl.Na 58.443 g/mol
2 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.5 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
15 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
16 NaHCO3
CHO3.Na 84.006 g/mol
10 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
17 Ethanol
C2H6O 46.069 g/mol
20 G_PER_L CHEBI:16236
ethanol
 Role: Solvating media; Properties: Defined component, Organic compound, Simple component
18 vitamin K1
C31H46O2 450.707 g/mol
0.1 G_PER_L CHEBI:18067
phylloquinone
 Role: Vitamin; Properties: Organic compound, Defined component, Solution
19 NaOH
HO.Na 39.997 g/mol
1 G_PER_L CHEBI:32145
sodium hydroxide
 Properties: Defined component, Inorganic compound, Solution
20 Hemin
C34H32ClFeN4O4 651.952 g/mol
50 G_PER_L CHEBI:50385
hemin
 Role: Growth factor; Properties: Organic compound, Defined component, Simple component

Solutions

Sal Solution*

Dose: 40 G_PER_L

Vitamin K1 solution**

Dose: 0.2 G_PER_L

See mediadive_2227_Vitamin_K1_solution.yaml for composition

Hemin solution***

Dose: 10 G_PER_L

See mediadive_5229_Hemin_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:59.264283Z togo-import Imported from TOGO Medium Source: NBRC, ID: M3290
2026-01-28T19:48:12.189362Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-09T08:04:20.110735+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T03:08:48.068314Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:20:08.261090Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009703
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.251673+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "modified PYG Medium" to "modified_pyg_medium" for consistent matching
2026-05-16T09:03:17.180532+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:40.477387+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:29.815224+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:50.332802+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:15.111004+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 88bbd893a643ce73462f41ab0ac16245b27f5a2d73504a9a8ef1f81bafed3884