brain_heart_infusion_agar_with_soil_extract (CultureMech:009715)

Overview

External ontology

TOGO:M337 — Brain Heart Infusion Agar With Soil Extract

Applications

Microbial cultivation

Synonyms

{'name': 'bhi_glucose_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:82', 'original_category': 'bacterial'}
{'name': 'bhi_glucose_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:82', 'original_category': 'bacterial'}
{'name': 'brain_heart_infusion_agar', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J28', 'original_category': 'bacterial'}
{'name': 'brain_heart_infusion_agar_with_2_nacl', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J263', 'original_category': 'bacterial'}

Notes

Source: https://togomedium.org/medium/M337 Original source: JCM - JCM_M342 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=342 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_009715["brain_heart_infusion_agar_with_soil_extract"]
    ing0_Calf_brains["Calf brains"]
    CultureMech_009715 --> ing0_Calf_brains
    ing1_Beef_heart["Beef heart"]
    CultureMech_009715 --> ing1_Beef_heart
    ing2_Proteose_peptone["Proteose peptone"]
    CultureMech_009715 --> ing2_Proteose_peptone
    ing3_Dextrose["Dextrose"]
    CultureMech_009715 --> ing3_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing3_Dextrose -.-> CHEBI_17634
    ing4_Sodium_chloride["Sodium chloride"]
    CultureMech_009715 --> ing4_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing4_Sodium_chloride -.-> CHEBI_26710
    ing5_Disodium_phosphate["Disodium phosphate"]
    CultureMech_009715 --> ing5_Disodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing5_Disodium_phosphate -.-> CHEBI_34683
    ing6_Glucose["Glucose"]
    CultureMech_009715 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_Agar["Agar"]
    CultureMech_009715 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509

Ingredients (8)

#	Ingredient	Concentration	Ontology	Notes
1	Calf brains Difco/BD Brain Heart Infusion 237500	variable VARIABLE	UBERON:0000955 brain	infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product
2	Beef heart Difco/BD Brain Heart Infusion 237500	variable VARIABLE	UBERON:0000948 heart	infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product
3	Proteose peptone Difco/BD Brain Heart Infusion 237500	10.0 G_PER_L	MICRO:0000180 (source link unavailable) proteose peptone	Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product
4	Dextrose C6H12O6 180.156 g/mol Difco/BD Brain Heart Infusion 237500	2.0 G_PER_L	CHEBI:17634 D-glucose	Carbon/energy source; Constituent of Brain Heart Infusion commercial product
5	Sodium chloride Cl.Na 58.443 g/mol Difco/BD Brain Heart Infusion 237500	5.0 G_PER_L	CHEBI:26710 sodium chloride	Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product
6	Disodium phosphate HO4P.2Na 141.958 g/mol Difco/BD Brain Heart Infusion 237500	2.5 G_PER_L	CHEBI:34683 disodium hydrogenphosphate	pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product
7	Glucose C6H12O6 180.156 g/mol	5 G_PER_L	CHEBI:17234 glucose
8	Agar	12 G_PER_L	CHEBI:2509 agar

Solutions

Soil extract (see Medium [M94])

Dose: 1 G_PER_L

Cross-reference to Medium M94

Target organisms

No target organisms are recorded.

Provenance

Merged from 6 source records.

KOMODO_82_BHI-GLUCOSE_medium (CultureMech:006566)
TOGO_M337_Brain_Heart_Infusion_Agar_With_Soil_Extract (CultureMech:009715)
bhi_glucose_medium (CultureMech:001983)
brain_heart_infusion_agar (CultureMech:002647)
brain_heart_infusion_agar_with_2_nacl (CultureMech:002621)
brain_heart_infusion_agar_with_soil_extract (CultureMech:002702)

Timestamp	Curator	Action	Notes
2026-01-27T06:35:54.871580Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M337
2026-01-28T19:48:22.346355Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:37.316708+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 1 solution(s) from ingredients
2026-03-13T06:20:08.328491Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009715
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name
2026-03-17T06:30:13.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.319063+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Brain Heart Infusion Agar With Soil Extract" to "brain_heart_infusion_agar_with_soil_extract" for consistent matching
2026-05-16T09:03:17.229515+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-06-05T07:40:40.526883+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:00.065685+00:00	recipe-merger	Merged 6 duplicate recipes into canonical record	Sources: KOMODO_82_BHI-GLUCOSE_medium, TOGO_M337_Brain_Heart_Infusion_Agar_With_Soil_Extract, bhi_glucose_medium, brain_heart_infusion_agar, brain_heart_infusion_agar_with_2_nacl, brain_heart_infusion_agar_with_soil_extract
2026-08-06T03:05:00.065764+00:00	merge_recipes.py	MERGED_RECIPES	merged 6 source recipe(s) on fingerprint a6b0678a3e68e60759e7d3c78e3f86ae7ab79094267d5658820bb396f7b6b3ae