Overview
- External ontology
- TOGO:M339 — HNW Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M339 Original source: JCM - JCM_M344 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=344
Composition
flowchart LR
CultureMech_009717["hnw_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009717 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NH4Cl["NH4Cl"]
CultureMech_009717 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_NaNO3["NaNO3"]
CultureMech_009717 --> ing2_NaNO3
CHEBI_63005["CHEBI:63005"]
ing2_NaNO3 -.-> CHEBI_63005
ing3_NaWO4_2H2O["NaWO4・2H2O"]
CultureMech_009717 --> ing3_NaWO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing3_NaWO4_2H2O -.-> CHEBI_63939
ing4_NaOH["NaOH"]
CultureMech_009717 --> ing4_NaOH
CHEBI_32145["CHEBI:32145"]
ing4_NaOH -.-> CHEBI_32145
ing5_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009717 --> ing5_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing5_Carbon_dioxide_gas -.-> CHEBI_16526
ing6_Hydrogen_gas["Hydrogen gas"]
CultureMech_009717 --> ing6_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing6_Hydrogen_gas -.-> CHEBI_18276
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
330 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
NaNO3
NO3.Na 84.994 g/mol
|
1 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
NaWO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 6 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 7 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: Gas |
Solutions
MJ(-N) synthetic seawater (see Medium [M260])
Dose: 670 G_PER_L
Cross-reference to Medium M260
8% NaHCO3 solution
Dose: 12.5 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M339_HNW_Medium (CultureMech:009717)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.873798Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M339 |
| 2026-01-28T19:48:09.589895Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:15.539795+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:20:08.339751Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009717 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.330073+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HNW Medium" to "hnw_medium" for consistent matching |
| 2026-05-16T09:03:17.241321+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:40.541229+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.355376+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:15.166100+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2dbfba97e6d61739f9d4d52c8460dde9747e2ac564c623b1c4fd464ed6f19a0c |