Overview
- External ontology
- TOGO:M342 — SME Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M342 Original source: JCM - JCM_M347 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=347
Composition
flowchart LR
CultureMech_009721["sme_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009721 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_009721 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Resazurin["Resazurin"]
CultureMech_009721 --> ing2_Resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Resazurin -.-> CHEBI_8806
ing3_Na2S_9H2O["Na2S・9H2O"]
CultureMech_009721 --> ing3_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing3_Na2S_9H2O -.-> CHEBI_76209
ing4__NH4_2SO4["(NH4)2SO4"]
CultureMech_009721 --> ing4__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing4__NH4_2SO4 -.-> CHEBI_62946
ing5_Sulfur__powder_["Sulfur (powder)"]
CultureMech_009721 --> ing5_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing5_Sulfur__powder_ -.-> CHEBI_33403
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009721 --> ing6_Yeast_extract__BD_Difco_
ing7_H2SO4["H2SO4"]
CultureMech_009721 --> ing7_H2SO4
CHEBI_26836["CHEBI:26836"]
ing7_H2SO4 -.-> CHEBI_26836
ing8_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009721 --> ing8_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing8_Carbon_dioxide_gas -.-> CHEBI_16526
ing9_N2["N2"]
CultureMech_009721 --> ing9_N2
CHEBI_17997["CHEBI:17997"]
ing9_N2 -.-> CHEBI_17997
ing10_Hydrogen_gas["Hydrogen gas"]
CultureMech_009721 --> ing10_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing10_Hydrogen_gas -.-> CHEBI_18276
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
500 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound |
| 4 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Simple component, Defined component, Inorganic compound |
| 6 | Sulfur (powder) | 10 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 | Yeast extract (BD-Difco) | 0.4 G_PER_L | Properties: Undefined component | |
| 8 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 9 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 10 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 11 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
Solutions
Synthetic seawater (2 x) (see Medium [M236])
Dose: 500 G_PER_L
Cross-reference to Medium M236
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M342_SME_Medium (CultureMech:009721)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:54.878391Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M342 |
| 2026-01-28T19:48:16.576770Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:27.380197+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:08.359771Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009721 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.349351+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SME Medium" to "sme_medium" for consistent matching |
| 2026-05-16T09:03:17.260266+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:40.564522+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.361869+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:15.195846+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f4fd2c7bbf70f0a14a364351c1df8133e51c67d7a82c9246dbdba9e548ff6c97 |