modified_sulfate_reducing_bacteria_medium

CultureMech:009727 bacterial COMPLEX LIQUID
Original name: Modified Sulfate Reducing Bacteria Medium

Overview

External ontology
TOGO:M348 — Modified Sulfate Reducing Bacteria Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M348 Original source: JCM - JCM_M354 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=354

Composition

flowchart LR
    CultureMech_009727["modified_sulfate_reducing_bacteria_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009727 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_009727 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_009727 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_009727 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_009727 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Resazurin["Resazurin"]
    CultureMech_009727 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_009727 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_009727 --> ing7_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_9H2O -.-> CHEBI_76209
    ing8_KCl["KCl"]
    CultureMech_009727 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_Sodium_acetate["Sodium acetate"]
    CultureMech_009727 --> ing9_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing9_Sodium_acetate -.-> CHEBI_32954
    ing10_Vitamin_B12["Vitamin B12"]
    CultureMech_009727 --> ing10_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing10_Vitamin_B12 -.-> CHEBI_176843
    ing11_Na2SO4["Na2SO4"]
    CultureMech_009727 --> ing11_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing11_Na2SO4 -.-> CHEBI_32149
    ing12_Na2WO4_2H2O["Na2WO4・2H2O"]
    CultureMech_009727 --> ing12_Na2WO4_2H2O
    CHEBI_63939["CHEBI:63939"]
    ing12_Na2WO4_2H2O -.-> CHEBI_63939
    ing13_PIPES["PIPES"]
    CultureMech_009727 --> ing13_PIPES
    CHEBI_39033["CHEBI:39033"]
    ing13_PIPES -.-> CHEBI_39033
    ing14_Na2SeO4["Na2SeO4"]
    CultureMech_009727 --> ing14_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing14_Na2SeO4 -.-> CHEBI_77775
    ing15_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_009727 --> ing15_Tryptone__BD_Difco_
    ing16_HCl["HCl"]
    CultureMech_009727 --> ing16_HCl
    CHEBI_17883["CHEBI:17883"]
    ing16_HCl -.-> CHEBI_17883
    ing17_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009727 --> ing17_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing17_Carbon_dioxide_gas -.-> CHEBI_16526
    ing18_N2["N2"]
    CultureMech_009727 --> ing18_N2
    CHEBI_17997["CHEBI:17997"]
    ing18_N2 -.-> CHEBI_17997
    ing19_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009727 --> ing19_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing19_Hydrogen_gas -.-> CHEBI_18276

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
2 NaCl
Cl.Na 58.443 g/mol
20 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.15 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
6 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
7 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
3 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component
9 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.166 G_PER_L CHEBI:32954
sodium acetate
 Role: Buffer; Properties: Organic compound, Defined component, Simple component
11 Vitamin B12 0.05 G_PER_L CHEBI:176843
vitamin B12
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
12 Na2SO4
2Na.O4S 142.043 g/mol
4 G_PER_L CHEBI:32149
sodium sulfate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13 Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
2 G_PER_L CHEBI:63939
sodium tungstate dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14 PIPES 3.46 G_PER_L CHEBI:39033
PIPES
 Role: Buffer; Properties: Organic compound, Defined component, Simple component
15 Na2SeO4
2Na.O4Se 188.936 g/mol
0.5 G_PER_L CHEBI:77775
sodium selenate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
16 Tryptone (BD-Difco) 0.5 G_PER_L Properties: Undefined component
17 HCl
HCl 36.461 g/mol
variable VARIABLE CHEBI:17883
hydrogen chloride
 Role: Solvating media; Properties: Defined component, Inorganic compound, Solution
18 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
19 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
20 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス

Solutions

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.885582Z togo-import Imported from TOGO Medium Source: JCM, ID: M348
2026-01-28T19:48:32.670285Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: HCl; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:04:54.247924+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:08.397075Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009727
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:34.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.385509+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Sulfate Reducing Bacteria Medium" to "modified_sulfate_reducing_bacteria_medium" for consistent matching
2026-05-16T09:03:17.293741+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:40.601098+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:34.093301+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:50.370309+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:15.209207+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint eacbf86276b7b8561c84c34eb88925d8ec36b9005b4377b4d5048cee7d58b17a