Overview
- External ontology
- TOGO:M350 — MJ Basal Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M350 Original source: JCM - JCM_M356 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=356
Composition
flowchart LR
CultureMech_009730["mj_basal_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_009730 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_NaHCO3["NaHCO3"]
CultureMech_009730 --> ing1_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing1_NaHCO3 -.-> CHEBI_32139
ing2_Na2S2O3_5H2O["Na2S2O3・5H2O"]
CultureMech_009730 --> ing2_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing2_Na2S2O3_5H2O -.-> CHEBI_32150
ing3_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009730 --> ing3_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing3_Carbon_dioxide_gas -.-> CHEBI_16526
ing4_Nitrogen_gas["Nitrogen gas"]
CultureMech_009730 --> ing4_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing4_Nitrogen_gas -.-> CHEBI_17997
ing5_Oxygen_gas["Oxygen gas"]
CultureMech_009730 --> ing5_Oxygen_gas
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
Na2S2O3・5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1.5 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Simple component |
| 4 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 5 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 6 | Oxygen gas | variable VARIABLE | Properties: ガス |
Solutions
MJ(-N) synthetic seawater (see Medium [M260])
Dose: 1 G_PER_L
Cross-reference to Medium M260
Trace vitamins (see Medium [M190])
Dose: 1 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M350_MJ_Basal_Medium (CultureMech:009730)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.891626Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M350 |
| 2026-01-28T19:48:41.301687Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Oxygen gas |
| 2026-03-09T08:05:09.578243+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:08.421541Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009730 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.409456+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MJ Basal Medium" to "mj_basal_medium" for consistent matching |
| 2026-05-16T09:03:17.319988+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:40.628863+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.382000+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:15.225605+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e641a0331d5b9eae50a5706eebad05bdec6a2fcf5b6a16513dbf6277ce488b7c |