Overview
- External ontology
- TOGO:M357 — PY4S Agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M357 Original source: JCM - JCM_M363 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=363
Composition
flowchart LR
CultureMech_009737["py4s_agar"]
ing0_8___w_v__Na2CO3["8% (w/v) Na2CO3"]
CultureMech_009737 --> ing0_8___w_v__Na2CO3
ing1_0_1___w_v__resazurin_Na["0.1% (w/v) resazurin-Na"]
CultureMech_009737 --> ing1_0_1___w_v__resazurin_Na
ing2_Distilled_water["Distilled water"]
CultureMech_009737 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_Cellobiose["Cellobiose"]
CultureMech_009737 --> ing3_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing3_Cellobiose -.-> CHEBI_17057
ing4_Maltose["Maltose"]
CultureMech_009737 --> ing4_Maltose
CHEBI_18167["CHEBI:18167"]
ing4_Maltose -.-> CHEBI_18167
ing5_Glucose["Glucose"]
CultureMech_009737 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Soluble_starch["Soluble starch"]
CultureMech_009737 --> ing6_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing6_Soluble_starch -.-> CHEBI_28017
ing7_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
CultureMech_009737 --> ing7_Bacto_agar__BD_Difco_
ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009737 --> ing8_Yeast_extract__BD_Difco_
ing9_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_009737 --> ing9_Trypticase_peptone__BD_BBL_
ing10_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_009737 --> ing10_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009737 --> ing11_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing11_Carbon_dioxide_gas -.-> CHEBI_16526
ing12_Nitrogen_gas["Nitrogen gas"]
CultureMech_009737 --> ing12_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing12_Nitrogen_gas -.-> CHEBI_17997
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 8% (w/v) Na2CO3 | 2.5 G_PER_L | Role: Buffer; Properties: Defined component, Inorganic compound, Solution | |
| 2 | 0.1% (w/v) resazurin-Na | 1 G_PER_L | Role: pH dependent redox indicator; Properties: Defined component, Solution | |
| 3 |
Distilled water
H2O 18.015 g/mol
|
850 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.25 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 5 |
Maltose
C12H22O11 342.297 g/mol
|
0.25 G_PER_L |
CHEBI:18167
alpha-maltose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
0.25 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 7 | Soluble starch | 0.25 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 8 | Bacto agar (BD-Difco) | 15 G_PER_L | Role: Solidifying component | |
| 9 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 10 | Trypticase peptone (BD-BBL) | 10 G_PER_L | Properties: Undefined component | |
| 11 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.3 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 12 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 13 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
Salt solution No. 1 (see Medium [M124])
Dose: 75 G_PER_L
Cross-reference to Medium M124
Salt solution No. 2 (see Medium [M124])
Dose: 75 G_PER_L
Cross-reference to Medium M124
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M357_PY4S_Agar (CultureMech:009737)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.904545Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M357 |
| 2026-01-28T19:48:14.567828Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:24.238743+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:08.485059Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009737 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.471699+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PY4S Agar" to "py4s_agar" for consistent matching |
| 2026-05-16T09:03:17.383347+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:40.694981+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:15.284892+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 475f464c35beb9606cbadcca514ff46f8d1053ad6aba479de4964416f8cf34f6 |