Overview
- External ontology
- TOGO:M377 — AGC2 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M377 Original source: JCM - JCM_M382 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=382
Composition
flowchart LR
CultureMech_009758["agc2_medium"]
ing0_yeast_extract["yeast extract"]
CultureMech_009758 --> ing0_yeast_extract
ing1_Sol__1["Sol. 1"]
CultureMech_009758 --> ing1_Sol__1
ing2_Sol__2["Sol. 2"]
CultureMech_009758 --> ing2_Sol__2
ing3_Distilled_water["Distilled water"]
CultureMech_009758 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
ing4_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009758 --> ing4_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_7H2O -.-> CHEBI_31795
ing5_K2HPO4["K2HPO4"]
CultureMech_009758 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_KCl["KCl"]
CultureMech_009758 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_009758 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_Ca_NO3_2["Ca(NO3)2"]
CultureMech_009758 --> ing8_Ca_NO3_2
CHEBI_64205["CHEBI:64205"]
ing8_Ca_NO3_2 -.-> CHEBI_64205
ing9_10_N_H2SO4["10 N H2SO4"]
CultureMech_009758 --> ing9_10_N_H2SO4
ing10_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_009758 --> ing10_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_7H2O -.-> CHEBI_75836
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 2 | Sol. 1 | 700 G_PER_L | ||
| 3 | Sol. 2 | 300 G_PER_L | ||
| 4 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Merged 2 duplicates: 700.0, 300.0] |
| 5 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 9 |
Ca(NO3)2
Ca.2NO3 164.086 g/mol
|
10 G_PER_L |
CHEBI:64205
calcium nitrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 10 | 10 N H2SO4 | 5 G_PER_L | Role: Mineral source; Properties: Defined component, Solution, Inorganic compound | |
| 11 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
44.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M377_AGC2_Medium (CultureMech:009758)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.943752Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M377 |
| 2026-01-28T19:48:18.913013Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:00.430756Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:08.658144Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009758 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.652253+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AGC2 Medium" to "agc2_medium" for consistent matching |
| 2026-06-05T07:40:40.873292+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:34.163952+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:30.134119+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.466678+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:15.492242+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d55b370a7798d7d260b1e5992ecb18ed91489706f6d6793314ccd03d20349ded |