mv_lactate_medium

CultureMech:009764 bacterial COMPLEX LIQUID
Original name: MV/Lactate Medium

Overview

External ontology
TOGO:M383 — MV/Lactate Medium
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'desulfovibrio_piger_medium', 'source': 'TOGO', 'source_id': 'TOGO:M396', 'original_category': 'bacterial'}
Notes
Source: https://togomedium.org/medium/M383 Original source: JCM - JCM_M388 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=388

Composition

flowchart LR
    CultureMech_009764["mv_lactate_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009764 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_009764 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_009764 --> ing2_Yeast_extract
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_009764 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_009764 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NH4Cl["NH4Cl"]
    CultureMech_009764 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Resazurin["Resazurin"]
    CultureMech_009764 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_009764 --> ing7_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_9H2O -.-> CHEBI_76209
    ing8_NaHCO3["NaHCO3"]
    CultureMech_009764 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_Na2SO4["Na2SO4"]
    CultureMech_009764 --> ing9_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing9_Na2SO4 -.-> CHEBI_32149
    ing10_Sodium_lactate["Sodium lactate"]
    CultureMech_009764 --> ing10_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing10_Sodium_lactate -.-> CHEBI_75228
    ing11_Na2S2O3_5H2O["Na2S2O3・5H2O"]
    CultureMech_009764 --> ing11_Na2S2O3_5H2O
    CHEBI_32150["CHEBI:32150"]
    ing11_Na2S2O3_5H2O -.-> CHEBI_32150
    ing12_N2["N2"]
    CultureMech_009764 --> ing12_N2
    CHEBI_17997["CHEBI:17997"]
    ing12_N2 -.-> CHEBI_17997

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
3 Yeast extract 1 G_PER_L Role: Nutrient source; Properties: Undefined component
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
5 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
6 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
7 Resazurin
C12H7NO4 229.191 g/mol
0.5 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
8 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
75 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Defined component, Inorganic compound, Simple component
9 NaHCO3
CHO3.Na 84.006 g/mol
2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Simple component, Defined component, Inorganic compound
10 Na2SO4
2Na.O4S 142.043 g/mol
2 G_PER_L CHEBI:32149
sodium sulfate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
11 Sodium lactate
C3H5O3.Na 112.06 g/mol
1 G_PER_L CHEBI:75228
sodium lactate
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
12 Na2S2O3・5H2O
5H2O.2Na.O3S2 248.186 g/mol
1 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
 Properties: Defined component, Inorganic compound, Simple component
13 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas

Solutions

FeCl2 solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Trace element solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-27T06:35:54.952898Z togo-import Imported from TOGO Medium Source: JCM, ID: M383
2026-01-28T19:48:39.427267Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-09T08:05:06.278760+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T06:20:08.700918Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009764
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.696211+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MV/Lactate Medium" to "mv_lactate_medium" for consistent matching
2026-05-16T09:03:17.590241+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:40.923309+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:30.173929+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:50.479960+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:15.545013+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: TOGO_M383_MV_Lactate_Medium, TOGO_M396_Desulfovibrio_Piger_Medium
2026-08-06T03:05:15.545054+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 4bf8b6c900dbf88b722d27d9e23b4d3ffea2770be3bec9188e56fc9260e84ff2