Overview
- External ontology
- TOGO:M395 — Desulfovibrio Magneticus Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M395 Original source: JCM - JCM_M399 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=399
Composition
flowchart LR
CultureMech_009777["desulfovibrio_magneticus_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009777 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NH4Cl["NH4Cl"]
CultureMech_009777 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_K2HPO4["K2HPO4"]
CultureMech_009777 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_009777 --> ing3_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing3_Sodium_pyruvate -.-> CHEBI_50144
ing4_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_009777 --> ing4_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing4_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing5_Sodium_fumarate["Sodium fumarate"]
CultureMech_009777 --> ing5_Sodium_fumarate
CHEBI_115156["CHEBI:115156"]
ing5_Sodium_fumarate -.-> CHEBI_115156
ing6_N2["N2"]
CultureMech_009777 --> ing6_N2
CHEBI_17997["CHEBI:17997"]
ing6_N2 -.-> CHEBI_17997
ing7_FeCl3_6H2O["FeCl3・6H2O"]
CultureMech_009777 --> ing7_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing7_FeCl3_6H2O -.-> CHEBI_86254
ing8_Quinic_acid["Quinic acid"]
CultureMech_009777 --> ing8_Quinic_acid
CHEBI_26493["CHEBI:26493"]
ing8_Quinic_acid -.-> CHEBI_26493
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
101.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 1.0, 100.0] |
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.06 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.44 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 5 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.05 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Organic compound, Simple component |
| 6 |
Sodium fumarate
C4H2O4.2Na 160.036 g/mol
|
0.58 G_PER_L |
CHEBI:115156
disodium fumarate
|
Properties: Defined component, Simple component, Organic compound |
| 7 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 8 |
FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.45 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 9 | Quinic acid | 0.19 G_PER_L |
CHEBI:26493
quinic acid
|
Role: Growth factor; Properties: Defined component, Organic compound, Simple component |
Solutions
Fe(III) quinate solution (see below)
Dose: 2 G_PER_L
Trace minerals (see Medium [M142])
Dose: 4 G_PER_L
Cross-reference to Medium M142
Trace vitamins (see Medium [M190])
Dose: 8 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M395_Desulfovibrio_Magneticus_Medium (CultureMech:009777)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.977356Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M395 |
| 2026-01-28T19:48:09.121076Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:14.656817+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:08:41.764544Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:08.816454Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009777 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.811366+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Desulfovibrio Magneticus Medium" to "desulfovibrio_magneticus_medium" for consistent matching |
| 2026-05-16T09:03:17.700850+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:41.035538+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.524417+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:15.676893+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint aa4b14b0a8024bd31693b839096a696f9865c778d5e6651334a8d926200dbb68 |