Overview
- External ontology
- TOGO:M414 — Modified Marine Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M414 Original source: JCM - JCM_M416 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=416
Composition
flowchart LR
CultureMech_009799["modified_marine_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009799 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009799 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_009799 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009799 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009799 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009799 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6_KCl["KCl"]
CultureMech_009799 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_009799 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_KNO3["KNO3"]
CultureMech_009799 --> ing8_KNO3
CHEBI_63043["CHEBI:63043"]
ing8_KNO3 -.-> CHEBI_63043
ing9_NaBr["NaBr"]
CultureMech_009799 --> ing9_NaBr
CHEBI_63004["CHEBI:63004"]
ing9_NaBr -.-> CHEBI_63004
ing10_SrCl2_6H2O["SrCl2・6H2O"]
CultureMech_009799 --> ing10_SrCl2_6H2O
CHEBI_36385["CHEBI:36385"]
ing10_SrCl2_6H2O -.-> CHEBI_36385
ing11_Na2SeO4["Na2SeO4"]
CultureMech_009799 --> ing11_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing11_Na2SeO4 -.-> CHEBI_77775
ing12_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009799 --> ing12_Yeast_extract__BD_Difco_
ing13_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_009799 --> ing13_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing13_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing14_DL__Sodium_malate["DL--Sodium malate"]
CultureMech_009799 --> ing14_DL__Sodium_malate
ing15_N2["N2"]
CultureMech_009799 --> ing15_N2
CHEBI_17997["CHEBI:17997"]
ing15_N2 -.-> CHEBI_17997
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.15 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
19 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.42 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
9 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
KNO3
K.NO3 101.102 g/mol
|
1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 10 |
NaBr
Br.Na 102.894 g/mol
|
0.05 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 11 |
SrCl2・6H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.02 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 12 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.02 G_PER_L |
CHEBI:77775
sodium selenate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 13 | Yeast extract (BD-Difco) | 0.2 G_PER_L | Properties: Undefined component | |
| 14 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.088 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Simple component, Defined component, Organic compound |
| 15 | DL--Sodium malate | 1.8 G_PER_L | Properties: Defined component, Simple component, Organic compound | |
| 16 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Solutions
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Trace mineral solution (see Medium [M409])
Dose: 10 G_PER_L
Cross-reference to Medium M409
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_marine_medium (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.014138Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M414 |
| 2026-01-28T19:48:12.096534Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:19.959368+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:08.972926Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009799 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.961462+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Marine Medium" to "modified_marine_medium" for consistent matching |
| 2026-05-16T09:04:25.655586+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:37.039664+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.886845+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:58:26.260051+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:08.703803+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:48.754414+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 447249baaf5564ece7973ebce17c5f379642bc7d0b873b1303f119d9ea7ecf05 |