methanolinea_medium

CultureMech:009810 archaea COMPLEX LIQUID
Original name: Methanolinea Medium

Overview

External ontology
TOGO:M424 — Methanolinea Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M424 Original source: JCM - JCM_M424 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=424

Composition

flowchart LR
    CultureMech_009810["methanolinea_medium"]
    ing0_L__cysteine_HCl_H2O["L--cysteine・HCl・H2O"]
    CultureMech_009810 --> ing0_L__cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing0_L__cysteine_HCl_H2O -.-> CHEBI_91248
    ing1_N2["N2"]
    CultureMech_009810 --> ing1_N2
    CHEBI_17997["CHEBI:17997"]
    ing1_N2 -.-> CHEBI_17997
    ing2_Distilled_water["Distilled water"]
    CultureMech_009810 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_009810 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_009810 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NH4Cl["NH4Cl"]
    CultureMech_009810 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Resazurin["Resazurin"]
    CultureMech_009810 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_009810 --> ing7_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_6H2O -.-> CHEBI_86345
    ing8_NaHCO3["NaHCO3"]
    CultureMech_009810 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_sodium_acetate["sodium acetate"]
    CultureMech_009810 --> ing9_sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing9_sodium_acetate -.-> CHEBI_32954
    ing10_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_009810 --> ing10_Yeast_extract__BD_Difco_
    ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009810 --> ing11_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing11_Carbon_dioxide_gas -.-> CHEBI_16526
    ing12_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009810 --> ing12_Nitrogen_gas
    ing12_Nitrogen_gas -.-> CHEBI_17997
    ing13_sodium_formate["sodium formate"]
    CultureMech_009810 --> ing13_sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing13_sodium_formate -.-> CHEBI_62965

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 L--cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
10 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Organic compound, Solution
2 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
3 Distilled water
H2O 18.015 g/mol
1000.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Merged 2 duplicates: 900.0, 100.0]
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.15 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5 KH2PO4
H2O4P.K 136.084 g/mol
0.14 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
6 NH4Cl
Cl.H4N 53.492 g/mol
0.54 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
7 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
8 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
9 NaHCO3
CHO3.Na 84.006 g/mol
2.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
10 sodium acetate
C2H3O2.Na 82.034 g/mol
0.1 G_PER_L CHEBI:32954
sodium acetate
 Role: Buffer; Properties: Defined component, Simple component, Organic compound
11 Yeast extract (BD-Difco) 0.1 G_PER_L Properties: Undefined component
12 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: Gas
13 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
14 sodium formate
CHO2.Na 68.007 g/mol
2.72 G_PER_L CHEBI:62965
sodium formate
 Properties: Defined component, Simple component, Inorganic compound

Solutions

Na2S・9H2O solutions

Dose: 10 G_PER_L

Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution

Solution A

Dose: 900 G_PER_L

See mediadive_5342_Solution_A.yaml for composition

Solution B

Dose: 100 G_PER_L

See mediadive_5343_Solution_B.yaml for composition

Trace vitamins solution (see Medium [M278])

Dose: 2 G_PER_L

Cross-reference to Medium M278

Trace element solution (see Medium [M278])

Dose: 1 G_PER_L

Cross-reference to Medium M278

Se/W solution (see Medium [M278])

Dose: 1 G_PER_L

Cross-reference to Medium M278

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:55.032786Z togo-import Imported from TOGO Medium Source: JCM, ID: M424
2026-01-28T19:48:17.051838Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2
2026-03-09T08:04:28.208263+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 6 solution(s) from ingredients
2026-03-13T03:08:56.973860Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:20:09.058285Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009810
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:28.043583+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Methanolinea Medium" to "methanolinea_medium" for consistent matching
2026-05-16T09:03:17.910053+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:41.260206+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:50.581493+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:27.494893+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint d25541921ef3e2b599b586fc2c93327b300a61c5ad3838cf21c79914bece0ad1