Overview
- External ontology
- TOGO:M431 — BN3 Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M431 Original source: JCM - JCM_M431 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=431
Composition
flowchart LR
CultureMech_009817["bn3_medium"]
ing0_Resazurin["Resazurin"]
CultureMech_009817 --> ing0_Resazurin
CHEBI_8806["CHEBI:8806"]
ing0_Resazurin -.-> CHEBI_8806
ing1_Na2S_9H2O["Na2S・9H2O"]
CultureMech_009817 --> ing1_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing1_Na2S_9H2O -.-> CHEBI_76209
ing2_NaHCO3["NaHCO3"]
CultureMech_009817 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_Vitamin_B12["Vitamin B12"]
CultureMech_009817 --> ing3_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing3_Vitamin_B12 -.-> CHEBI_176843
ing4_Sulfur__powder_["Sulfur (powder)"]
CultureMech_009817 --> ing4_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing4_Sulfur__powder_ -.-> CHEBI_33403
ing5_Glucose["Glucose"]
CultureMech_009817 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009817 --> ing6_Yeast_extract__BD_Difco_
ing7_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_009817 --> ing7_Tryptone__BD_Difco_
ing8_Basal_medium__see_below_["Basal medium (see below)"]
CultureMech_009817 --> ing8_Basal_medium__see_below_
ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009817 --> ing9_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing9_Carbon_dioxide_gas -.-> CHEBI_16526
ing10_Nitrogen_gas["Nitrogen gas"]
CultureMech_009817 --> ing10_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing10_Nitrogen_gas -.-> CHEBI_17997
ing11_Distilled_water["Distilled water"]
CultureMech_009817 --> ing11_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing11_Distilled_water -.-> CHEBI_15377
ing12_NaCl["NaCl"]
CultureMech_009817 --> ing12_NaCl
CHEBI_26710["CHEBI:26710"]
ing12_NaCl -.-> CHEBI_26710
ing13_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009817 --> ing13_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing13_CaCl2_2H2O -.-> CHEBI_86158
ing14_KH2PO4["KH2PO4"]
CultureMech_009817 --> ing14_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing14_KH2PO4 -.-> CHEBI_63036
ing15_NH4Cl["NH4Cl"]
CultureMech_009817 --> ing15_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing15_NH4Cl -.-> CHEBI_31206
ing16_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009817 --> ing16_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing16_MgCl2_6H2O -.-> CHEBI_86345
ing17_KCl["KCl"]
CultureMech_009817 --> ing17_KCl
CHEBI_32588["CHEBI:32588"]
ing17_KCl -.-> CHEBI_32588
ing18_25__HCl["25% HCl"]
CultureMech_009817 --> ing18_25__HCl
ing19_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_009817 --> ing19_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_2H2O -.-> CHEBI_75213
ing20_H3BO3["H3BO3"]
CultureMech_009817 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_009817 --> ing21_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing21_FeSO4_7H2O -.-> CHEBI_75836
ing22_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_009817 --> ing22_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing22_MnCl2_4H2O -.-> CHEBI_86368
ing23_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_009817 --> ing23_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing23_CoCl2_6H2O -.-> CHEBI_35696
ing24_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_009817 --> ing24_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing24_NiCl2_6H2O -.-> CHEBI_34887
ing25_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_009817 --> ing25_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing25_ZnSO4_7H2O -.-> CHEBI_32312
ing26_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_009817 --> ing26_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing26_CuCl2_2H2O -.-> CHEBI_86318
ing27_Na2SeO3_5H2O["Na2SeO3・5H2O"]
CultureMech_009817 --> ing27_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing27_Na2SeO3_5H2O -.-> CHEBI_131361
ing28_Na2WO4_2H2O["Na2WO4・2H2O"]
CultureMech_009817 --> ing28_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing28_Na2WO4_2H2O -.-> CHEBI_63939
ing29_NaOH["NaOH"]
CultureMech_009817 --> ing29_NaOH
CHEBI_32145["CHEBI:32145"]
ing29_NaOH -.-> CHEBI_32145
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 2 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.36 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
3.4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 | Vitamin B12 | 5 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 5 | Sulfur (powder) | 20 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
3.6 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 7 | Yeast extract (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 8 | Tryptone (BD-Difco) | 1.5 G_PER_L | Properties: Undefined component | |
| 9 | Basal medium (see below) | 1 G_PER_L | ||
| 10 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 11 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 12 |
Distilled water
H2O 18.015 g/mol
|
989.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 987.0, 1.0] |
| 13 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.075 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 | 25% HCl | 12.5 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 20 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
36 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
30 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
2.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
190 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
Na2SeO3・5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
6 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
8 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Simple component, Inorganic compound |
Solutions
Trace element solution (see below)
Dose: 1 G_PER_L
Selenite--tungstate solution (see below)
Dose: 1 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M431_BN3_Medium (CultureMech:009817)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:55.043130Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M431 |
| 2026-01-28T19:48:20.777758Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:34.470052+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:04.033637Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:09.110766Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009817 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.091714+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BN3 Medium" to "bn3_medium" for consistent matching |
| 2026-05-16T09:03:17.960268+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:41.321827+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.606040+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:16.004888+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5e6246bf2d2af35b2f30b2bf49d58458d05e30fd27bb25d7b428c0044b7a70c9 |