Overview
- External ontology
- TOGO:M438 — RT2 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M438 Original source: JCM - JCM_M438 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=438
Composition
flowchart LR
CultureMech_009823["rt2_medium"]
ing0_Sol__1["Sol. 1"]
CultureMech_009823 --> ing0_Sol__1
ing1_Distilled_water["Distilled water"]
CultureMech_009823 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_Yeast_extract["Yeast extract"]
CultureMech_009823 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_009823 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009823 --> ing4_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_2H2O -.-> CHEBI_86158
ing5_KH2PO4["KH2PO4"]
CultureMech_009823 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_NH4Cl["NH4Cl"]
CultureMech_009823 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_Resazurin["Resazurin"]
CultureMech_009823 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009823 --> ing8_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_6H2O -.-> CHEBI_86345
ing9_KCl["KCl"]
CultureMech_009823 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10_Na2SO4["Na2SO4"]
CultureMech_009823 --> ing10_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing10_Na2SO4 -.-> CHEBI_32149
ing11_CAPSO["CAPSO"]
CultureMech_009823 --> ing11_CAPSO
CHEBI_183882["CHEBI:183882"]
ing11_CAPSO -.-> CHEBI_183882
ing12_NaOH["NaOH"]
CultureMech_009823 --> ing12_NaOH
CHEBI_32145["CHEBI:32145"]
ing12_NaOH -.-> CHEBI_32145
ing13_N2["N2"]
CultureMech_009823 --> ing13_N2
CHEBI_17997["CHEBI:17997"]
ing13_N2 -.-> CHEBI_17997
ing14_Sodium_lactate["Sodium lactate"]
CultureMech_009823 --> ing14_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing14_Sodium_lactate -.-> CHEBI_75228
ing15_Sodium_dithionite["Sodium dithionite"]
CultureMech_009823 --> ing15_Sodium_dithionite
CHEBI_66870["CHEBI:66870"]
ing15_Sodium_dithionite -.-> CHEBI_66870
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sol. 1 | 902 G_PER_L | ||
| 2 |
Distilled water
H2O 18.015 g/mol
|
1010.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 3 duplicates: 900.0, 100.0, 10.0] |
| 3 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Simple component, Defined component, Inorganic compound |
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound |
| 9 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 11 |
Na2SO4
2Na.O4S 142.043 g/mol
|
4 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 12 |
CAPSO
C9H19NO4S 237.321 g/mol
|
11.86 G_PER_L |
CHEBI:183882
3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid
|
Role: Buffer; Properties: Simple component, Defined component |
| 13 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 14 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 15 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
2.24 G_PER_L |
CHEBI:75228
sodium lactate
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 16 |
Sodium dithionite
2Na.O4S2 174.11 g/mol
|
0.1 G_PER_L |
CHEBI:66870
sodium dithionite
|
Properties: Defined component, Inorganic compound, Simple component |
Solutions
sodium lactate solution
Dose: 100 G_PER_L
sodium dithionite solution
Dose: 10 G_PER_L
Trace metal solution (see Medium [M288])
Dose: 1 G_PER_L
Cross-reference to Medium M288
Selenite--tungstate solution (see Medium [M431])
Dose: 1 G_PER_L
Cross-reference to Medium M431
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M438_RT2_Medium (CultureMech:009823)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.058932Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M438 |
| 2026-01-28T19:48:27.377042Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:45.391674+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:15.850039Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:09.174360Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009823 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.151284+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RT2 Medium" to "rt2_medium" for consistent matching |
| 2026-05-16T09:03:18.026519+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:41.391234+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.633331+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:16.127427+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b6c696c8584c388da2d4faba87d1d504623f439db38962ddfaada56cf77a55bf |